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Journal of Clinical Microbiology, June 1999, p. 1892-1898, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Genetic Features of Streptococcus agalactiae Strains Causing Severe Neonatal Infections, as Revealed by Pulsed-Field Gel Electrophoresis and hylB Gene Analysis

Karine Rolland, Corinne Marois, Veronique Siquier, Blandine Cattier, and Roland Quentin*

Département de Microbiologie Médicale et Moléculaire, Unité de Bactériologie, Centre Hospitalier Universitaire Bretonneau, 37044 Tours, France

Received 22 September 1998/Returned for modification 10 January 1999/Accepted 20 March 1999

A collection of 114 independent Streptococcus agalactiae strains, including 54 strains isolated from the cerebrospinal fluid (CSF) samples of neonates and 60 strains from asymptomatic patients, was characterized by pulsed-field gel electrophoresis (PFGE) of DNA restricted with SmaI and by PCR analysis of the hylB gene. All strains were previously studied by multilocus enzyme electrophoresis (MLEE) (R. Quentin, H. Huet, F.-S. Wang, P. Geslin, A. Goudeau, and R. K. Selander, J. Clin. Microbiol. 33:2576-2581, 1995). Among these 114 strains, there were 92 PFGE patterns. Eleven genetic groups (A to K) were identified with 38% divergence. A more homogeneous group (PFGE group A) was defined, consisting of 73% of the strains previously identified as belonging to a particular MLEE phylogenetic group. A 162-kb fragment was identified as a marker of strains that invaded the central nervous system of neonates. It was detected in 69% of the PFGE patterns obtained with CSF isolates and in only 1.8% of the PFGE patterns obtained with carrier strains. The hylB gene encoding hyaluronate lyase was amplified for all strains in our collection. Ten of 15 isolates belonging to an MLEE subgroup, previously described as being likely to cause invasive infection, had an insertion in the hylB gene (IS1548).

* Corresponding author. Mailing address: Département de Microbiologie Médicale et Moléculaire, Unité de Bactériologie, CHRU Bretonneau, 2 Bd. Tonnellé, 37044 Tours Cedex, France. Phone: (33) 2 47 47 80 56. Fax: (33) 2 47 47 38 12. E-mail: quentin{at}pop.med.univ-tours.fr.

Journal of Clinical Microbiology, June 1999, p. 1892-1898, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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