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Journal of Clinical Microbiology, June 1999, p. 1932-1934, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of Three Nucleic Acid Amplification Methods for Direct Detection of Mycobacterium tuberculosis Complex in Respiratory Specimens

S. X. Wang and L. Tay*

Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Singapore, Republic of Singapore

Received 27 August 1998/Returned for modification 28 December 1998/Accepted 20 March 1999

Two hundred thirty respiratory specimens from 230 patients were analyzed by using COBAS AMPLICOR PCR, Amplified Mycobacterium tuberculosis Direct Test, and ligase chain reaction methods. Results were compared with those of smear microscopy and radiometric culture (Bactec) methods. No significant differences were observed among the results of the three methods, which are acceptable for direct detection of M. tuberculosis complex in respiratory specimens.

* Corresponding author. Mailing address: Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608, Republic of Singapore. Phone: (65)-3214906. Fax: (65)-2226826. E-mail: gpttay{at}sgh.gov.sg.

Journal of Clinical Microbiology, June 1999, p. 1932-1934, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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