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Journal of Clinical Microbiology, June 1999, p. 1977-1979, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of the ImmunoCardSTAT! Rotavirus Assay for Detection of Group A Rotavirus in Fecal Specimens

Penelope H. Dennehy,1,* Michele Hartin,2 Sara M. Nelson,1 and Shirley F. Reising3

Division of Pediatric Infectious Diseases, Rhode Island Hospital, and Department of Pediatrics, Brown University School of Medicine, Providence, Rhode Island1; Clinical Laboratory, Department of Pathology, Children's Hospital, San Diego, California2; and Microbiology Laboratory, Children's Hospital Medical Center and Research Foundation, Cincinnati, Ohio3

Received 1 June 1998/Returned for modification 24 August 1998/Accepted 25 February 1999

Rapid detection of group A rotavirus was performed by using ImmunoCardStat! Rotavirus (ICS-RV) (which uses immunogold-based, horizontal-flow membrane technology), two commercial enzyme immunoassays (Premier Rotaclone and TestPack Rotavirus), and electron microscopy. A total of 249 stool specimens collected from children with gastroenteritis between February and April 1997 were tested. After resolution of 19 of the 22 discordant results by reverse transcription-PCR for group A rotavirus, ICS-RV detected 125 positives while Rotaclone and TestPack detected 127 and 129 positives, respectively. The sensitivity, specificity, positive predictive value, and negative predictive value were 94.0, 100, 100, and 93.4% for ICS-RV; 95.5, 100, 100, and 95.0% for Rotaclone; and 97.0, 96.5, 97.0, and 96.5% for TestPack. ICS-RV was sensitive and specific and was relatively simple to perform and interpret.

* Corresponding author. Mailing address: Division of Pediatric Infectious Diseases, Rhode Island Hospital, 593 Eddy St., Providence, RI 02903. Phone: (401) 444-4298. Fax: (401) 444-5650. E-mail: Penelope_Dennehy{at}brown.edu.

Journal of Clinical Microbiology, June 1999, p. 1977-1979, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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