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Journal of Clinical Microbiology, June 1999, p. 1985-1993, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Medically Relevant Trichosporon Species Based on Sequences of Internal Transcribed Spacer Regions and Construction of a Database for Trichosporon Identification

Takashi Sugita,1 Akemi Nishikawa,2 Reiko Ikeda,1 and Takako Shinoda1,*

Department of Microbiology1 and Department of Immunobiology,2 Meiji Pharmaceutical University, Kiyose, Tokyo, Japan

Received 16 October 1998/Returned for modification 14 January 1999/Accepted 1 March 1999

The nucleotide sequences of the internal transcribed spacer (ITS) 1 and 2 regions in the rRNA gene were determined by directly sequencing PCR-amplified fragments for all of the species (17 species and five varieties) in the genus Trichosporon. Comparative sequence analysis suggests that six medically relevant species, T. asahii, T. asteroides, T. cutaneum, T. inkin, T. mucoides, and T. ovoides, can be readily identified by their ITS sequences. In addition, the sequence analysis showed that conspecific strains have fewer than 1% nucleotide differences in the ITS 1 and 2 regions overall. Molecular phylogenetic trees are also presented.

* Corresponding author. Mailing address: Department of Microbiology, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo, 204-8588 Japan. Phone: 81-424-95-8761. Fax: 81-424-95-8761. E-mail: shinoda{at}my-pharm.ac.jp.

Journal of Clinical Microbiology, June 1999, p. 1985-1993, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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