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Journal of Clinical Microbiology, June 1999, p. 2010-2012, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Toscana Virus-Specific Immunoglobulins G and M by an Enzyme-Linked Immunosorbent Assay Based on Recombinant Viral Nucleoprotein

Maria Grazia Ciufolini,* Cristiano Fiorentini, Paola di Bonito, Stefania Mochi, and Colomba Giorgi

Laboratory of Virology, Istituto Superiore di Sanità, 00161 Rome, Italy

Received 25 September 1998/Returned for modification 11 December 1998/Accepted 4 March 1999

An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.

* Corresponding author. Mailing address: Laboratory of Virology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: (3906) 49903235. Fax: (3906) 49902082. E-mail: ciufolin{at}virus1.net.iss.it.

Journal of Clinical Microbiology, June 1999, p. 2010-2012, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.