This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rastogi, N.
Right arrow Articles by Berchel, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rastogi, N.
Right arrow Articles by Berchel, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 1999, p. 2016-2019, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Species-Specific Identification of Mycobacterium leprae by PCR-Restriction Fragment Length Polymorphism Analysis of the hsp65 Gene

Nalin Rastogi,* Khye Seng Goh, and Mylene Berchel

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, 97165 Pointe à Pitre Cedex, Guadeloupe

Received 22 January 1999/Returned for modification 15 February 1999/Accepted 3 March 1999

PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene present in all mycobacteria was used in the present investigation to characterize Mycobacterium leprae. Bacilli were extracted and purified from different organs from experimentally infected armadillos and nude mice (Swiss mice of nu/nu origin). A total of 15 samples were assayed in duplicate, and the results were compared with those obtained for a total of 147 cultivable mycobacteria representing 34 species. Irrespective of its origin or viability, M. leprae strains from all the samples were uniformly characterized by two fragments of 315 and 135 bp upon BstEII digestion and two fragments of 265 and 130 bp upon HaeIII digestion. PRA is a relatively simple method and permits the conclusive identification of M. leprae to the species level.

* Corresponding author. Mailing address: Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Morne Jolivière B. P. 484, 97165 Pointe à Pitre Cedex, Guadeloupe. Phone: 590-893-881. Fax: 590-893-880. E-mail: rastogi{at}ipagua.gp.

Journal of Clinical Microbiology, June 1999, p. 2016-2019, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lini, N., Shankernarayan, N. P., Dharmalingam, K. (2009). Quantitative real-time PCR analysis of Mycobacterium leprae DNA and mRNA in human biopsy material from leprosy and reactional cases. J Med Microbiol 58: 753-759 [Abstract] [Full Text]  
  • Chen, H.-J., Tsai, J.-C., Chang, T.-C., Hung, W.-C., Tseng, S.-P., Hsueh, P.-R., Teng, L.-J. (2008). PCR-RFLP assay for species and subspecies differentiation of the Streptococcus bovis group based on groESL sequences. J Med Microbiol 57: 432-438 [Abstract] [Full Text]  
  • Tsai, J.-C., Hsueh, P.-R., Lin, H.-M., Chang, H.-J., Ho, S.-W., Teng, L.-J. (2005). Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region. J. Clin. Microbiol. 43: 235-241 [Abstract] [Full Text]  
  • Hoffmann, H., Roggenkamp, A. (2003). Population Genetics of the Nomenspecies Enterobacter cloacae. Appl. Environ. Microbiol. 69: 5306-5318 [Abstract] [Full Text]  
  • Konomi, N., Lebwohl, E., Mowbray, K., Tattersall, I., Zhang, D. (2002). Detection of Mycobacterial DNA in Andean Mummies. J. Clin. Microbiol. 40: 4738-4740 [Abstract] [Full Text]  
  • Ucko, M., Colorni, A., Kvitt, H., Diamant, A., Zlotkin, A., Knibb, W. R. (2002). Strain Variation in Mycobacteriummarinum Fish Isolates. Appl. Environ. Microbiol. 68: 5281-5287 [Abstract] [Full Text]  
  • da Silva Rocha, A., Werneck Barreto, A. M., Dias Campos, C. E., Villas-Boas da Silva, M., Fonseca, L., Saad, M. H., Degrave, W. M., Suffys, P. N. (2002). Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of hsp65. J. Clin. Microbiol. 40: 4191-4196 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Tsai, J.-C., Chen, P.-W., Hsu, J.-C., Lai, H.-C., Lee, C.-N., Ho, S.-W. (2002). groESL Sequence Determination, Phylogenetic Analysis, and Species Differentiation for Viridans Group Streptococci. J. Clin. Microbiol. 40: 3172-3178 [Abstract] [Full Text]  
  • Teng, L.-J., Hsueh, P.-R., Wang, Y.-H., Lin, H.-M., Luh, K.-T., Ho, S.-W. (2001). Determination of Enterococcus faecalis groESL Full-Length Sequence and Application for Species Identification. J. Clin. Microbiol. 39: 3326-3331 [Abstract] [Full Text]  
  • Brunello, F., Ligozzi, M., Cristelli, E., Bonora, S., Tortoli, E., Fontana, R. (2001). Identification of 54 Mycobacterial Species by PCR-Restriction Fragment Length Polymorphism Analysis of the hsp65 Gene. J. Clin. Microbiol. 39: 2799-2806 [Abstract] [Full Text]  
  • Kim, B.-J., Lee, K.-H., Park, B.-N., Kim, S.-J., Bai, G.-H., Kim, S.-J., Kook, Y.-H. (2001). Differentiation of Mycobacterial Species by PCR-Restriction Analysis of DNA (342 Base Pairs) of the RNA Polymerase Gene (rpoB). J. Clin. Microbiol. 39: 2102-2109 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»