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Journal of Clinical Microbiology, June 1999, p. 2020-2023, Vol. 37, No. 6
0095-1137/99/$04.00+0

Typing of Bovine Viral Diarrhea Viruses Directly from Blood of Persistently Infected Cattle by Multiplex PCR

S. A. Gilbert, K. M. Burton, S. E. Prins, and D. Deregt*

Animal Diseases Research Institute, Canadian Food Inspection Agency, Lethbridge, Alberta, Canada

Received 29 July 1998/Returned for modification 5 January 1999/Accepted 25 February 1999

A nested multiplex PCR was developed for genotyping of bovine viral diarrhea viruses (BVDVs). The assay could detect as little as 3 50% tissue culture infective doses of BVDV per ml and typed 42 out of 42 cell culture isolates. BVDV was also successfully typed, with or without RNA extraction, from all 27 whole-blood samples examined from 22 carriers or probable carriers and 5 experimentally infected cattle.

* Corresponding author. Mailing address: Animal Diseases Research Institute, P.O. Box 640, Lethbridge, Alberta, Canada T1J 3Z4. Phone: (403) 382-5500. Fax: (403) 381-1202. E-mail: deregtd{at}em.agr.ca.

Journal of Clinical Microbiology, June 1999, p. 2020-2023, Vol. 37, No. 6
0095-1137/99/$04.00+0


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