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Journal of Clinical Microbiology, June 1999, p. 2024-2026, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Times to Detection of Bacteria and Yeasts in BACTEC 9240 Blood Culture Bottles

Barbara S. Reisner* and Gail L. Woods

Department of Pathology, University of Texas Medical Branch, Galveston, Texas 77555-0740

Received 18 December 1998/Returned for modification 8 February 1999/Accepted 2 March 1999

A 7-day incubation protocol was instituted with the BACTEC 9240 system for a 1-year period to determine the times to detection of clinically relevant organisms. A total of 23,686 blood and 693 sterile body fluid cultures were received; some cultures were held longer by special request. Of 1,609 likely skin contaminants, 42 were recovered on day 5, 34 on day 6, 16 on day 7, and 5 on day 8. Of 2,803 usual pathogens, 34 were recovered on day 5, 24 on day 6, 15 on day 7 and 1 on day 8. Twenty-one of the latter organisms were considered significant laboratory isolates because they were the first isolates from the respective patients. Chart review showed that 10 of 21 were considered clinically significant, but only 3 (all yeasts) affected the treatment of the patient. Our data show that 4 days of incubation were sufficient to recover all clinically relevant bacteria and 6 days were required to recover all clinically relevant yeasts.

* Corresponding author. Mailing address: University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555-0740. Phone: (409) 772-1120. Fax: (409) 772-5683. E-mail: breisner{at}utmb.edu.

Journal of Clinical Microbiology, June 1999, p. 2024-2026, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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