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Journal of Clinical Microbiology, June 1999, p. 2053-2057, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Amplified-Fragment Length Polymorphism Analysis versus Macro-Restriction Fragment Analysis for Molecular Typing of Streptococcus pneumoniae Isolates

Johan van Eldere,1,* Paul Janssen,1 Annette Hoefnagels-Schuermans,1 Stefaan van Lierde,2 and Willy E. Peetermans3

Departments of Microbiology and Immunology,1 Pediatrics,2 and Internal Medicine,3 Infectious Diseases Research Group, Rega Institute for Medical Research, Katholieke Universiteit Leuven, B-3000 Leuven, Belgium

Received 23 December 1998/Returned for modification 6 February 1999/Accepted 20 March 1999

Forty-eight pneumococci were genotyped by on-line laser fluorescence amplified-fragment length polymorphism (AFLP) and pulsed-field gel electrophoresis (PFGE) analysis of chromosomal restriction fragments. Overall, the data generated by the two methods corresponded well. However, with AFLP, clusters were delineated at a higher similarity level, and isolate differentiation was more pronounced. AFLP and PFGE were equally efficient for assessing intraserotype diversity. We conclude that AFLP is a useful alternative to PFGE.

* Corresponding author. Mailing address: Rega Institute for Medical Research, Katholieke Universiteit Leuven, Minderbroederstraat 10, B-3000 Leuven, Belgium. Phone: 32-16-33 73 72. Fax: 32-16-33 73 40. E-mail: johan.vaneldere{at}rega.kuleuven.ac.be.

Journal of Clinical Microbiology, June 1999, p. 2053-2057, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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