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Journal of Clinical Microbiology, June 1999, p. 2093-2098, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

PCR Amplification from Fixed Tissue Indicates Frequent Involvement of Brachyspira aalborgi in Human Intestinal Spirochetosis

Andrew S. J. Mikosza,1 Tom La,1 C. Josephine Brooke,1 Christian F. Lindboe,2 Peter B. Ward,3 Ralf G. Heine,3 John G. Guccion,4 W. Bastiaan de Boer,5 and David J. Hampson1,*

Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150,1 Department of Microbiology and Infectious Diseases, Royal Children's Hospital, Parkville, Victoria 3052,3 and Department of Anatomical Pathology, PathCentre, Nedlands, Western Australia 6009,5 Australia; Department of Pathology, Vest-Agder County Central Hospital, N-4604 Kristiansand, Norway2; and Pathology and Laboratory Medicine Service, Department of Veterans Affairs Medical Center, Washington, D.C.4

Received 30 October 1998/Returned for modification 17 December 1998/Accepted 17 March 1999

PCR procedures amplifying portions of the 16S rRNA and NADH oxidase genes of Brachyspira aalborgi and Serpulina pilosicoli were applied to DNA extracted from paraffin-embedded human colonic or rectal tissues from 30 Norwegian, Australian, and U.S. patients, 16 of whom had histologic evidence of intestinal spirochetosis (IS). B. aalborgi-specific sequences were identified by PCR in 10 of the IS patients (62.5%) but none of the others, while S. pilosicoli sequences were not detected in tissues from any patient. Direct sequencing of products from three of the positive samples provided further confirmation of the presence of B. aalborgi. B. aalborgi may be a more common cause of intestinal spirochetosis than has been previously thought.

* Corresponding author. Mailing address: Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia. Phone: 61 8 9360 2287. Fax: 61 8 9310 4144. E-mail: hampson{at}numbat.murdoch.edu.au.

Journal of Clinical Microbiology, June 1999, p. 2093-2098, Vol. 37, No. 6
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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