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Journal of Clinical Microbiology, July 1999, p. 2142-2147, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Subtyping of Haemophilus influenzae Strains by Pulsed-Field Gel Electrophoresis

Mitsumasa Saito,* Akiko Umeda, and Shin-ichi Yoshida

Department of Bacteriology, Faculty of Medicine, Kyushu University, Fukuoka 812-8582, Japan

Received 23 November 1998/Returned for modification 11 January 1999/Accepted 29 March 1999

A total of 200 isolates of Haemophilus influenzae were analyzed by serotyping, biotyping, and pulsed-field gel electrophoresis (PFGE). A total of 178 epidemiologically unrelated strains of H. influenzae demonstrated a variety of genome patterns by PFGE, and 165 genotypes were thus obtained in this study. PFGE typing proved to have a much stronger discriminatory power than either serotyping or biotyping. Six serotype b strains were all classified into discrete genotypes. A PFGE analysis of 18 strains obtained from the nasopharynx, blood, and cerebrospinal fluid of patients with meningitis also supported the hypothesis that invasive H. influenzae disseminates from the nasopharynx to the bloodstream and then subsequently to other body sites. PFGE typing of 10 other strains isolated from household contacts of patients with H. influenzae infection revealed that the strain that caused the H. influenzae infection often colonized the nasopharynges of household contacts. Our findings suggest that PFGE analysis is useful for the epidemiological study of H. influenzae infection, even when the invasive disease is caused by serotype b strains.

* Corresponding author. Mailing address: Department of Bacteriology, Faculty of Medicine, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-6130. Fax: 81-92-642-6133. E-mail: msaito{at}bact.med.kyushu-u.ac.jp.

Journal of Clinical Microbiology, July 1999, p. 2142-2147, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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