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Journal of Clinical Microbiology, July 1999, p. 2326-2329, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Prenatal Diagnosis of Parvovirus B19-Induced Hydrops Fetalis by Chemiluminescence In Situ Hybridization

M. Musiani,1,* P. Pasini,2 M. Zerbini,1 G. Gentilomi,1 A. Roda,2 G. Gallinella,1 E. Manaresi,1 and S. Venturoli1

Division of Microbiology, Department of Clinical and Experimental Medicine,1 and Department of Pharmaceutical Sciences,2 University of Bologna, Bologna, Italy

Received 25 January 1999/Returned for modification 1 March 1999/Accepted 5 April 1999

Parvovirus B19 can be transmitted transplacentally from the infected mother to the fetus during pregnancy, and hydrops fetalis, abortion, or stillbirth can result. In our study we explored the use of chemiluminescence in situ hybridization to detect B19 DNA on cord blood cells, amniotic fluid cells, and pleuric fluid cells from several cases of hydrops fetalis. B19 DNA was detected by using digoxigenin-labeled probes immunoenzymatically visualized with the chemiluminescent adamantil-1,2-dioxetane phenyl phosphate substrate for alkaline phosphatase. The luminescent signal emitted from the hybridized probes was detected, analyzed, and measured with a high-performance, low-light-level imaging luminograph connected to an optical microscope and to a personal computer for the quantification and localization of the chemiluminescent emission inside individual cells.

* Corresponding author. Mailing address: Division of Microbiology, Department of Clinical and Experimental Medicine, Via Massarenti 9, 40138 Bologna, Italy. Phone: 0039 51 302435. Fax: 0039 51 341632. E-mail: musiani{at}med.unibo.it.

Journal of Clinical Microbiology, July 1999, p. 2326-2329, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.




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