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Journal of Clinical Microbiology, July 1999, p. 2330-2332, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Amplification of a 500-Base-Pair Fragment from Cultured Isolates of Mycobacterium bovis

Juan Germán Rodríguez,1,* Juan Carlos Fissanoti,2 Patricia Del Portillo,1 Manuel Elkin Patarroyo,3 María Isabel Romano,2 and Angel Cataldi2

Corporación CorpoGen,1 and Instituto de Inmunología, Hospital San Juan de Dios,3 Santafé de Bogotá, D.C. Colombia, and Instituto de Biotecnología CICV/INTA, Morón, Argentina2

Received 25 November 1998/Returned for modification 20 January 1999/Accepted 20 March 1999

The presence of a 500-bp fragment which amplifies a region from the genome of Mycobacterium bovis (J. G. Rodriguez, G. A. Meija, P. Del Portillo, M. E. Patarroyo, and L. A. Murillo, Microbiology 141:2131-2138, 1995) was evaluated by carrying out PCR on 121 M. bovis isolates. The M. bovis strains, previously characterized by culture and biochemical tests, were isolated from cattle in different regions of Argentina, Mexico, and Colombia. Four additional strains isolated from sea lions that belong to the M. tuberculosis complex were also included in the study. All of the isolates tested were PCR positive, rendering the expected 500-bp band and giving a correlation of 100% with previous microbiological characterization. Southern blot analysis revealed a common band of 1,800 bp and a polymorphic high-molecular-mass hybridization pattern. The results show that this assay may be useful for diagnosis and identification of M. bovis in cattle.

* Corresponding author. Mailing address: Corporación CorpoGen, Calle 26 A No. 37-28, Santafé de Bogotá, D.C. Colombia. Phone: 57-1-368-5411. Fax: 57-1-3684987. E-mail: corpogen{at}colomsat.net.co.

Journal of Clinical Microbiology, July 1999, p. 2330-2332, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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