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Journal of Clinical Microbiology, July 1999, p. 2346-2347, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Detection of Respiratory Viruses by Shell Vial Assay Using Simultaneous Culture of HEp-2, LLC-MK2, and MDCK Cells in a Single Vial

José M. Navarro-Marí,* Sara Sanbonmatsu-Gámez, Mercedes Pérez-Ruiz, and Manuel De La Rosa-Fraile

Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, 18014 Granada, Spain

Received 25 November 1998/Returned for modification 4 March 1999/Accepted 26 March 1999

A shell vial assay with simultaneous culture of HEp-2, LLC-MK2, and MDCK cell lines in a single tube (CoHLM SV assay) was compared with traditional tube culture (TC) for the detection of the main respiratory viruses in 358 nasal wash specimens. A total of 170 strains were isolated from 168 virus-positive samples. A total of 94.1% of the strains (160 strains; 128 respiratory syncytial viruses and 32 other viruses) were detected by the CoHLM SV assay in 48 h, whereas 98.2% of the strains (167 strains; 132 respiratory syncytial viruses and 35 other viruses) were detected by TC in a mean time of 6 days. The CoHLM SV assay may be useful for the rapid detection of respiratory viruses.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Virgen de las Nieves, Avda. Fuerzas Armadas, s/n, 18014 Granada, Spain. Phone: 0034 958 241109. Fax: 0034 958 241282. E-mail: delarosa{at}cica.es.

Journal of Clinical Microbiology, July 1999, p. 2346-2347, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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