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Journal of Clinical Microbiology, July 1999, p. 2350-2351, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Inactivation of Mycobacterium tuberculosis for DNA Typing Analysis

P. Bemer-Melchior* and H. B. Drugeon

Department of Microbiology, Centre Hospitalier Universitaire, Nantes, France

Received 30 October 1998/Returned for modification 7 February 1999/Accepted 26 March 1999

DNA fingerprinting analysis of Mycobacterium tuberculosis is used for epidemiological studies and the control of laboratory cross-contamination. Because standardized procedures are not entirely safe for mycobacteriology laboratory staff, the paper proposes a new technique for the processing of specimens. The technique ensures the inactivation of M. tuberculosis before DNA extraction without the loss of DNA integrity. The control of inactivated cultures should be rigorous and should involve the use of two different culture media incubated for at least 4 months.

* Corresponding author. Mailing address: Laënnec Hospital, Bd. J. Monod, 44093 Saint-Herblain cedex 1, France. Phone: (33) 2-40-16-54-57. Fax: (33) 2-40-16-54-55. E-mail: hdrugeon{at}chu-nantes.fr.

Journal of Clinical Microbiology, July 1999, p. 2350-2351, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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