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Journal of Clinical Microbiology, July 1999, p. 2354-2357, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Clonal Diversity among Recently Emerged Strains of Vibrio parahaemolyticus O3:K6 Associated With Pandemic Spread

Prasanta K. Bag,1 Suvobroto Nandi,2 Rupak K. Bhadra,2 T. Ramamurthy,1 S. K. Bhattacharya,1 M. Nishibuchi,3 T. Hamabata,4 Shinji Yamasaki,4 Yoshifumi Takeda,4 and G. Balakrish Nair1,*

National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700 010,1 and Indian Institute of Chemical Biology, Calcutta-700 032,2 India, and Center for Southeast Asian Studies, Kyoto University, Yoshida, Kyoto 606-01,3 and Research Institute, International Medical Center of Japan, Shinjuku-ku, Tokyo 162-8655,4 Japan

Received 23 November 1998/Returned for modification 27 February 1999/Accepted 26 March 1999

The genomes of the O3:K6 strains of Vibrio parahaemolyticus which abruptly emerged in Calcutta, India, in February 1996 and which demonstrated an unusual potential to spread and an enhanced propensity to cause infections were examined by different molecular techniques to determine clonality. No restriction fragment length polymorphism (RFLP) in the gene encoding the thermostable direct hemolysin was observed among the O3:K6 isolates of V. parahaemolyticus. Clonal diversity among the O3:K6 strains became evident by examining the RFLPs of the rrn operons and by the use of pulsed-field gel electrophoresis. Five ribotypes were distinguished among the O3:K6 strains examined, with ribotype R4 constituting the major type. Strains of O3:K6 isolated between June and August 1996 showed different pulsotypes compared to the pulsotypes of strains isolated before and after this period, indicating genetic reassortment among these strains, but those isolated between August 1996 and March 1998 showed identical or nearly similar pulsotypes. It is clear that there is a certain degree of genomic reassortment among the O3:K6 clones but that these strains are predominantly one clone.

* Corresponding author. Mailing address: National Institute of Cholera and Enteric Diseases, P-33, CIT Road Scheme XM, Beliaghata, Calcutta-700 010, India. Phone: 91-33-350-4598. Fax: 91-33-3505066. E-mail: krishgb{at}giasc101.vsnl.net.in.

Journal of Clinical Microbiology, July 1999, p. 2354-2357, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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