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Journal of Clinical Microbiology, July 1999, p. 2366-2368, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of an Epstein-Barr Virus (EBV) Immunoglobulin M Enzyme-Linked Immunosorbent Assay Using a Synthetic Convergent Peptide Library, or Mixotope, for Diagnosis of Primary EBV Infection

D. Tranchand-Bunel,1,* H. Gras-Masse,2 B. Bourez,3 L. Dedecker,3 and C. Auriault1

Laboratoire d'Immunologie Cellulaire, CNRS UMR 8527,1 Laboratoire de Synthèse, Structure et Fonction des Biomolécules, CNRS UMR 8525,2 and Laboratoire d'Immunologie-Sérologie, Département Biologie Spécialisée,3 Institut Pasteur de Lille, 59019 Lille, France

Received 21 September 1998/Returned for modification 19 November 1998/Accepted 16 April 1999

An immunoglobulin M (IgM) enzyme-linked immunosorbent assay (ELISA) was developed by using a 24-amino-acid peptide of the 18-kDa Epstein-Barr virus (EBV) viral capsid antigen (VCAp18). IgM detection was increased by 23% by using this antigen. Detection of IgM antibodies to the EBV proteins in the new ELISA was 100% specific and 95% sensitive.

* Corresponding author. Mailing address: Laboratoire d'Immunologie Cellulaire, UMR 8527, Institut Pasteur de Lille, 1 rue du Professeur Calmette, BP 245, 59019 Lille Cédex, France. Phone: 0320-87-12-43. Fax: 0320-87-12-33. E-mail: d.bunel{at}infobiogen.fr.

Journal of Clinical Microbiology, July 1999, p. 2366-2368, Vol. 37, No. 7
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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