This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marshall, S.
Right arrow Articles by Johnson, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marshall, S.
Right arrow Articles by Johnson, W. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 1999, p. 2473-2478, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Comparison of Molecular Methods for Typing Vibrio parahaemolyticus

S. Marshall,1 C. G. Clark,2,* G. Wang,1 M. Mulvey,1 M. T. Kelly,3 and W. M. Johnson1,2

National Laboratory for Enteric Pathogens2 and National Laboratory for Outbreak Investigations and Antibiotic Resistance,1 Laboratory Centre for Disease Control, Health Canada, Ottawa, Ontario, and Provincial Laboratory, B.C. Centre for Disease Control, Vancouver, British Columbia,3 Canada

Received 18 November 1998/Returned for modification 3 March 1999/Accepted 27 April 1999

An outbreak of Vibrio parahaemolyticus gastroenteritis on Canada's west coast in 1997 emphasized the need to develop molecular methods for differentiation and typing of these organisms. Isolates were analyzed by enterobacterial repetitive intergenic consensus sequence (ERIC) PCR, detection of restriction fragment length polymorphisms (RFLP) in rRNA genes (ribotyping), pulsed-field gel electrophoresis (PFGE), and RFLP analysis of the genetic locus encoding the polar flagellum (Fla locus RFLP analysis). ERIC PCR and ribotyping were the most informative typing methods, especially when used together, while Fla locus RFLP analysis was the least discriminatory. PFGE exhibited good discrimination but suffered from a high incidence of DNA degradation. ERIC PCR and ribotyping will be useful for the evaluation of genetic and epidemiological relationships among V. parahaemolyticus strains.

* Corresponding author. Mailing address: Laboratory Centre for Disease Control, Bureau of Microbiology, Health Canada, Federal Laboratories, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2094. Fax: (204) 789-2097. E-mail: Clifford_Clark{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, August 1999, p. 2473-2478, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Harth-Chu, E., Espejo, R. T., Christen, R., Guzman, C. A., Hofle, M. G. (2009). Multiple-Locus Variable-Number Tandem-Repeat Analysis for Clonal Identification of Vibrio parahaemolyticus Isolates by Using Capillary Electrophoresis. Appl. Environ. Microbiol. 75: 4079-4088 [Abstract] [Full Text]  
  • Banerjee, S. K., Farber, J. M. (2009). Susceptibility of Vibrio parahaemolyticus to Tris-Dependent DNA Degradation during Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 47: 870-871 [Full Text]  
  • Kalpoe, J. S., Templeton, K. E., Horrevorts, A. M., Endtz, H. P., Kuijper, E. J., Bernards, A. T., Klaassen, C. H. W. (2007). Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA, Pulsed-Field Gel Electrophoresis, and Amplified Fragment Length Polymorphism Analyses. J. Clin. Microbiol. 45: 4048-4050 [Abstract] [Full Text]  
  • Shima, K., Terajima, J., Sato, T., Nishimura, K., Tamura, K., Watanabe, H., Takeda, Y., Yamasaki, S. (2004). Development of a PCR-Restriction Fragment Length Polymorphism Assay for the Epidemiological Analysis of Shiga Toxin-Producing Escherichia coli. J. Clin. Microbiol. 42: 5205-5213 [Abstract] [Full Text]  
  • Wong, H.-c., Chen, S.-Y., Chen, M.-Y., Oliver, J. D., Hor, L.-I, Tsai, W.-C. (2004). Pulsed-Field Gel Electrophoresis Analysis of Vibrio vulnificus Strains Isolated from Taiwan and the United States. Appl. Environ. Microbiol. 70: 5153-5158 [Abstract] [Full Text]  
  • Okura, M., Osawa, R., Iguchi, A., Arakawa, E., Terajima, J., Watanabe, H. (2003). Genotypic Analyses of Vibrio parahaemolyticus and Development of a Pandemic Group-Specific Multiplex PCR Assay. J. Clin. Microbiol. 41: 4676-4682 [Abstract] [Full Text]  
  • DePaola, A., Ulaszek, J., Kaysner, C. A., Tenge, B. J., Nordstrom, J. L., Wells, J., Puhr, N., Gendel, S. M. (2003). Molecular, Serological, and Virulence Characteristics of Vibrio parahaemolyticus Isolated from Environmental, Food, and Clinical Sources in North America and Asia. Appl. Environ. Microbiol. 69: 3999-4005 [Abstract] [Full Text]  
  • Sanchez, S., McCrackin Stevenson, M. A., Hudson, C. R., Maier, M., Buffington, T., Dam, Q., Maurer, J. J. (2002). Characterization of Multidrug-Resistant Escherichia coli Isolates Associated with Nosocomial Infections in Dogs. J. Clin. Microbiol. 40: 3586-3595 [Abstract] [Full Text]  
  • Yeung, P. S. M., Hayes, M. C., DePaola, A., Kaysner, C. A., Kornstein, L., Boor, K. J. (2002). Comparative Phenotypic, Molecular, and Virulence Characterization of Vibrio parahaemolyticus O3:K6 Isolates. Appl. Environ. Microbiol. 68: 2901-2909 [Abstract] [Full Text]  
  • Klaassen, C. H. W., van Haren, H. A., Horrevorts, A. M. (2002). Molecular Fingerprinting of Clostridium difficile Isolates: Pulsed-Field Gel Electrophoresis versus Amplified Fragment Length Polymorphism. J. Clin. Microbiol. 40: 101-104 [Abstract] [Full Text]  
  • Wong, H.-C., Lin, C.-H. (2001). Evaluation of Typing of Vibrioparahaemolyticus by Three PCR Methods Using Specific Primers. J. Clin. Microbiol. 39: 4233-4240 [Abstract] [Full Text]  
  • Okatani, A. T., Uto, T., Taniguchi, T., Horisaka, T., Horikita, T., Kaneko, K.-I., Hayashidani, H. (2001). Pulsed-Field Gel Electrophoresis in Differentiation of Erysipelothrix Species Strains. J. Clin. Microbiol. 39: 4032-4036 [Abstract] [Full Text]  
  • Masseret, E, Boudeau, J, Colombel, J F, Neut, C, Desreumaux, P, Joly, B, Cortot, A, Darfeuille-Michaud, A (2001). Genetically related Escherichia coli strains associated with Crohn's disease. Gut 48: 320-325 [Abstract] [Full Text]  
  • Tasteyre, A., Karjalainen, T., Avesani, V., Delmée, M., Collignon, A., Bourlioux, P., Barc, M.-C. (2000). Phenotypic and Genotypic Diversity of the Flagellin Gene (fliC) among Clostridium difficile Isolates from Different Serogroups. J. Clin. Microbiol. 38: 3179-3186 [Abstract] [Full Text]  
  • Römling, U., Tümmler, B. (2000). Achieving 100% Typeability of Pseudomonas aeruginosa by Pulsed-Field Gel Electrophoresis. J. Clin. Microbiol. 38: 464-465 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»