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Journal of Clinical Microbiology, August 1999, p. 2483-2487, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Novel Human Erythrovirus Associated with Transient Aplastic Anemia

Quang Tri Nguyen,1,* Christophe Sifer,1 Véronique Schneider,2 Xavier Allaume,1 Annabelle Servant,1 Françoise Bernaudin,3 Véronique Auguste,1 and Antoine Garbarg-Chenon1

Laboratoire de Virologie, Hôpital Armand Trousseau (EA 2391 UFR Saint-Antoine),1 and Laboratoire de Virologie, Hôpital Rothschild (EA 2391 UFR Saint-Antoine),2 75 571 Paris Cedex 12, and Service de Pédiatrie, Centre Hospitalier Intercommunal de Créteil, 94 010 Créteil Cedex,3 France

Received 21 December 1998/Returned for modification 15 April 1999/Accepted 6 May 1999

Erythrovirus (formerly parvovirus) B19 causes a wide range of diseases in humans, including anemia due to aplastic crisis. Diagnosis of B19 infection relies on serology and the detection of viral DNA by PCR. These techniques are usually thought to detect all erythrovirus field isolates, since the B19 genome is known to undergo few genetic variations. We have detected an erythrovirus (V9) markedly different from B19 in the serum and bone marrow of a child with transient aplastic anemia. The B19 PCR assay yielded a product that hybridized only very weakly to the B19-specific probe and whose sequence diverged more from those of 24 B19 viruses (11 to 14%) than the divergence found within the B19 group (<= 6.65%). Restriction enzyme analysis of the V9 genome revealed that this genetic divergence extended beyond the amplified region. Interestingly, serological tests failed to demonstrate a response characteristic of acute B19 infection. V9 could be a new erythrovirus, and new diagnostic tests are needed for its detection.

* Corresponding author. Mailing address: Institut Pasteur, Unité de Génétique et Biochimie du Développement, 25 rue du Dr. Roux, 75 724 Paris Cedex 15, France. Phone: 33 1 45 68 85 65. Fax: 33 1 40 61 34 40. E-mail: nqt{at}pasteur.fr.

Journal of Clinical Microbiology, August 1999, p. 2483-2487, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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