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Journal of Clinical Microbiology, August 1999, p. 2488-2492, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Value of Enterobacterial Repetitive Intergenic Consensus PCR for Study of Pasteurella multocida Strains Isolated from Mouths of Dogs

Julien Loubinoux,1 Alain Lozniewski,2 Christine Lion,2 Daniel Garin,3 Michèle Weber,2 and Alain E. Le Faou1,*

Laboratoire de Virologie, CHU Nancy-Brabois, 54511 Vandoeuvre-lès-Nancy Cédex,1 Laboratoire de Bactériologie, CHU Nancy, 54035 Nancy Cédex,2 and Service de Biologie Médicale, HIA Legouest, 57000 Metz,3 France

Received 10 November 1998/Returned for modification 20 January 1999/Accepted 25 April 1999

Fifty-six Pasteurella multocida strains (40 P. multocida subsp. septica and 16 P. multocida subsp. multocida strains) isolated from the mouths of 56 dogs among the 134 living in a French canine military training center (132e Groupe Cynophile de l'Armée de Terre, Suippes, France) were studied by use of enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) and restriction fragment length polymorphism (RFLP) techniques. Both techniques showed genomic heterogeneity of the strains studied. However, RFLP was more discriminatory than ERIC-PCR for differentiating P. multocida strains. All but three pairs of strains were discriminated by RFLP, suggesting a limited circulation of strains between these dogs living in proximity. Although ERIC-PCR is easier and faster to perform, it cannot be recommended for epidemiological studies of P. multocida strains.

* Corresponding author. Mailing address: Laboratoire de Virologie, CHU Nancy-Brabois, Route de Neufchâteau, 54511 Vandoeuvre-lès-Nancy Cédex, France. Phone: (33) 3 83 15 34 69. Fax: (33) 3 83 15 34 74. E-mail: a.lefaou{at}chu-nancy.fr.

Journal of Clinical Microbiology, August 1999, p. 2488-2492, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Meacham, K. J., Zhang, L., Foxman, B., Bauer, R. J., Marrs, C. F. (2003). Evaluation of Genotyping Large Numbers of Escherichia coli Isolates by Enterobacterial Repetitive Intergenic Consensus-PCR. J. Clin. Microbiol. 41: 5224-5226 [Abstract] [Full Text]  


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