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Journal of Clinical Microbiology, August 1999, p. 2564-2567, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Inhibition Enzyme-Linked Immunosorbent Assay for Serotyping of Group B Streptococcal Isolates

Gayathri Arakere,1,* Aurea E. Flores,2 Patricia Ferrieri,2 and Carl E. Frasch1

Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland,1 and Departments of Laboratory Medicine and Pathology and Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota2

Received 30 March 1999/Returned for modification 4 May 1999/Accepted 18 May 1999

Group B Streptococcus (GBS) is one of the most common organisms causing neonatal sepsis as well as serious infections in adults. Serotyping the organism is important in studying the epidemiology of the disease as well as deciding a course of treatment. There are several methods available for serotyping. Most of them need high-titered sera and are not quantitative. We are reporting a new inhibition enzyme-linked immunosorbent assay (ELISA) for serotyping which is sensitive and specific compared to the conventional methods but does not need high-titered serotype-specific antisera, as the specificity is controlled by the polysaccharide coating on the ELISA plates. The method can also be quantitative, and we have measured polysaccharide elaborated by different serotype V strains. Thus, the inhibition ELISA method will be useful in serotyping for epidemiological studies, assessing virulence, and performing strain selection for vaccine production.

* Corresponding author. Mailing address: Division of Bacterial Products, Center for Biologics Evaluation and Research, 1401 Rockville Pike, Mailstop HFM-428, Rockville, MD 20853. Phone: (301) 496-9173. Fax: (301) 402-2776. E-mail: arakereg{at}cber.fda.gov.

Journal of Clinical Microbiology, August 1999, p. 2564-2567, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 1999 by the American Society for Microbiology. All rights reserved.