This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Arakere, G.
Right arrow Articles by Frasch, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arakere, G.
Right arrow Articles by Frasch, C. E.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 1999, p. 2564-2567, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Inhibition Enzyme-Linked Immunosorbent Assay for Serotyping of Group B Streptococcal Isolates

Gayathri Arakere,1,* Aurea E. Flores,2 Patricia Ferrieri,2 and Carl E. Frasch1

Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland,1 and Departments of Laboratory Medicine and Pathology and Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota2

Received 30 March 1999/Returned for modification 4 May 1999/Accepted 18 May 1999

Group B Streptococcus (GBS) is one of the most common organisms causing neonatal sepsis as well as serious infections in adults. Serotyping the organism is important in studying the epidemiology of the disease as well as deciding a course of treatment. There are several methods available for serotyping. Most of them need high-titered sera and are not quantitative. We are reporting a new inhibition enzyme-linked immunosorbent assay (ELISA) for serotyping which is sensitive and specific compared to the conventional methods but does not need high-titered serotype-specific antisera, as the specificity is controlled by the polysaccharide coating on the ELISA plates. The method can also be quantitative, and we have measured polysaccharide elaborated by different serotype V strains. Thus, the inhibition ELISA method will be useful in serotyping for epidemiological studies, assessing virulence, and performing strain selection for vaccine production.

* Corresponding author. Mailing address: Division of Bacterial Products, Center for Biologics Evaluation and Research, 1401 Rockville Pike, Mailstop HFM-428, Rockville, MD 20853. Phone: (301) 496-9173. Fax: (301) 402-2776. E-mail: arakereg{at}cber.fda.gov.

Journal of Clinical Microbiology, August 1999, p. 2564-2567, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Wen, L., Wang, Q., Li, Y., Kong, F., Gilbert, G. L., Cao, B., Wang, L., Feng, L. (2006). Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus (Streptococcus agalactiae). J. Clin. Microbiol. 44: 1447-1452 [Abstract] [Full Text]  
  • Manning, S. D., Lacher, D. W., Davies, H. D., Foxman, B., Whittam, T. S. (2005). DNA Polymorphism and Molecular Subtyping of the Capsular Gene Cluster of Group B Streptococcus. J. Clin. Microbiol. 43: 6113-6116 [Abstract] [Full Text]  
  • Borchardt, S. M., Foxman, B., Chaffin, D. O., Rubens, C. E., Tallman, P. A., Manning, S. D., Baker, C. J., Marrs, C. F. (2004). Comparison of DNA Dot Blot Hybridization and Lancefield Capillary Precipitin Methods for Group B Streptococcal Capsular Typing. J. Clin. Microbiol. 42: 146-150 [Abstract] [Full Text]  
  • Slotved, H.-C., Elliott, J., Thompson, T., Konradsen, H. B. (2003). Latex Assay for Serotyping of Group B Streptococcus Isolates. J. Clin. Microbiol. 41: 4445-4447 [Abstract] [Full Text]  
  • Kong, F., Gowan, S., Martin, D., James, G., Gilbert, G. L. (2002). Serotype Identification of Group B Streptococci by PCR and Sequencing. J. Clin. Microbiol. 40: 216-226 [Abstract] [Full Text]  
  • Sellin, M., Olofsson, C., Håkansson, S., Norgren, M. (2000). Genotyping of the Capsule Gene Cluster (cps) in Nontypeable Group B Streptococci Reveals Two Major cps Allelic Variants of Serotypes III and VII. J. Clin. Microbiol. 38: 3420-3428 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»