This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yang, C.
Right arrow Articles by Lal, R. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yang, C.
Right arrow Articles by Lal, R. B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 1999, p. 2581-2586, Vol. 37, No. 8
0095-1137/99/$04.00+0

Detection of Phylogenetically Diverse Human Immunodeficiency Virus Type 1 Groups M and O from Plasma by Using Highly Sensitive and Specific Generic Primers

Chunfu Yang,1 Danuta Pieniazek,1 Sherry M. Owen,1 Carol Fridlund,1 John Nkengasong,2 Timothy D. Mastro,3,4,5 Mark A. Rayfield,5 Robert Downing,5 Benon Biryawaho,5 Amilcar Tanuri,6 Leopold Zekeng,7 Guido van der Groen,8 Feng Gao,9 and Renu B. Lal1,*

Project RETRO-CI, Abidjan, Ivory Coast2; HIV/AIDS Collaboration, Nonthaburi, Thailand3; HIV and Retrovirology Branch, Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases,1 and International Activities Branch, Division of HIV/AIDS Prevention-Surveillance and Epidemiology, National Center for HIV, STD and TB Prevention,4 Centers for Disease Control and Prevention, Atlanta, Georgia; Uganda Virus Research Institute, Entebbe, Uganda5; Laboratorio de Virologica Molecular, Departmento de Genetica, Rio de Janeiro, Brazil6; Center Hospitalier et Universitaire, Université de Yaoundé, Yaoundé, Cameroon7; Division of Microbiology, Institute of Tropical Medicine, Antwerp, Belgium8; and Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama9

Received 5 February 1999/Returned for modification 7 April 1999/Accepted 26 April 1999

The high degree of genetic diversity within human immunodeficiency virus type 1 (HIV-1), which includes two major groups, M (major) and O (outlier), and various env subtypes within group M (subtypes A to J), has made designing assays that will detect all known HIV-1 strains difficult. We have developed a generic primer set based on the conserved immunodominant region of transmembrane protein gp41 that can reliably amplify as few as 10 copies/PCR of viral DNA from near-full-length clones representing group M subtypes A to H (subtypes I and J were not available). The assay is highly sensitive in detecting plasma viral RNA from HIV-1 strains of diverse geographic origins representing different subtypes of HIV-1 group M as well as HIV-1 group O. Of the 253 group M plasma specimens (subtypes A, 68 specimens; B, 71; C, 19; D, 27; E, 23; F, 33; and G, 12), 250 (98.8%) were amplified by using the gp41 M/O primer set. More importantly, all 32 (100%) group O plasma samples were also amplified with these primers. In vitro spiking experiments further revealed that the assay could reliably detect as few as 25 copies/ml of viral RNA and gave positive signals in HIV-1-seropositive specimens with plasma copy numbers below the limits of detection by all commercially available viral load assays. In addition, analysis of five seroconversion panels indicated that the assay is highly sensitive for early detection of plasma viremia during the "window period." Thus, the highly sensitive assay will be useful for early detection of HIV-1 in clinical specimens from all known HIV-1 infections, regardless of their genotypes and geographic origins.

* Corresponding author. Mailing address: HIV and Retrovirology Branch, DASTLR/NCID, Centers for Disease Control and Prevention, Mail Stop D-12, 1600 Clifton Road, Atlanta, GA 30333. Phone: (404) 639-1036. Fax: (404) 639-2660. E-mail: rbl3{at}cdc.gov.

Journal of Clinical Microbiology, August 1999, p. 2581-2586, Vol. 37, No. 8
0095-1137/99/$04.00+0


This article has been cited by other articles:

  • Zhang, Q., Wang, L., Jiang, Y., Fang, L., Pan, P., Gong, S., Yao, J., Tang, Y.-W., Vermund, S. H., Jia, Y. (2008). Early Infant Human Immunodeficiency Virus Type 1 Detection Suitable for Resource-Limited Settings with Multiple Circulating Subtypes by Use of Nested Three-Monoplex DNA PCR and Dried Blood Spots. J. Clin. Microbiol. 46: 721-726 [Abstract] [Full Text]  
  • OUMA, C., KELLER, C. C., OPONDO, D. A., WERE, T., OTIENO, R. O., OTIENO, M. F., ORAGO, A. S. S., ONG'ECHA, J. M., VULULE, J. M., FERRELL, R. E., PERKINS, D. J. (2006). ASSOCIATION OF FC{gamma} RECEPTOR IIA (CD32) POLYMORPHISM WITH MALARIAL ANEMIA AND HIGH-DENSITY PARASITEMIA IN INFANTS AND YOUNG CHILDREN. Am J Trop Med Hyg 74: 573-577 [Abstract] [Full Text]  
  • Zouhair, S., Roussin-Bretagne, S., Moreau, A., Brunet, S., Laperche, S., Maniez, M., Barin, F., Harzic, M. (2006). Group O Human Immunodeficiency Virus Type 1 Infection That Escaped Detection in Two Immmunoassays. J. Clin. Microbiol. 44: 662-665 [Abstract] [Full Text]  
  • Gaudy, C., Moreau, A., Brunet, S., Descamps, J.-M., Deleplanque, P., Brand, D., Barin, F. (2004). Subtype B Human Immunodeficiency Virus (HIV) Type 1 Mutant That Escapes Detection in a Fourth-Generation Immunoassay for HIV Infection. J. Clin. Microbiol. 42: 2847-2849 [Abstract] [Full Text]  
  • Price, D. A., Scullard, G., Oxenius, A., Braganza, R., Beddows, S. A., Kazmi, S., Clarke, J. R., Johnson, G. E., Weber, J. N., Phillips, R. E. (2003). Discordant Outcomes following Failure of Antiretroviral Therapy Are Associated with Substantial Differences in Human Immunodeficiency Virus-Specific Cellular Immunity. J. Virol. 77: 6041-6049 [Abstract] [Full Text]  
  • Masciotra, S., Yang, C., Pieniazek, D., Thomas, C., Owen, S. M., McClure, H. M., Lal, R. B. (2002). Detection of Simian Immunodeficiency Virus in Diverse Species and of Human Immunodeficiency Virus Type 2 by Using Consensus Primers within the pol Region. J. Clin. Microbiol. 40: 3167-3171 [Abstract] [Full Text]  
  • Trask, S. A., Derdeyn, C. A., Fideli, U., Chen, Y., Meleth, S., Kasolo, F., Musonda, R., Hunter, E., Gao, F., Allen, S., Hahn, B. H. (2002). Molecular Epidemiology of Human Immunodeficiency Virus Type 1 Transmission in a Heterosexual Cohort of Discordant Couples in Zambia. J. Virol. 76: 397-405 [Abstract] [Full Text]  
  • Koch, W. H., Sullivan, P. S., Roberts, C., Francis, K., Downing, R., Mastro, T. D., Nkengasong, J., Hu, D., Masciotra, S., Schable, C., Lal, R. B. (2001). Evaluation of United States-Licensed Human Immunodeficiency Virus Immunoassays for Detection of Group M Viral Variants. J. Clin. Microbiol. 39: 1017-1020 [Abstract] [Full Text]  
  • Masciotra, S., Rudolph, D. L., van der Groen, G., Yang, C., Lal, R. B. (2000). Serological Detection of Infection with Diverse Human and Simian Immunodeficiency Viruses Using Consensus env Peptides. CVI 7: 706-709 [Abstract] [Full Text]  
  • Dorn, J., Masciotra, S., Yang, C., Downing, R., Biryahwaho, B., Mastro, T. D., Nkengasong, J., Pieniazek, D., Rayfield, M. A., Hu, D. J., Lal, R. B. (2000). Analysis of Genetic Variability within the Immunodominant Epitopes of Envelope gp41 from Human Immunodeficiency Virus Type 1 (HIV-1) Group M and Its Impact on HIV-1 Antibody Detection. J. Clin. Microbiol. 38: 773-780 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»