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Journal of Clinical Microbiology, August 1999, p. 2592-2597, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR-Restriction Enzyme Analysis

Sylvia Cardoso Leão,1,* Marcelo R. S. Briones,1 Marcelo Palma Sircili,1 Simone Carvalho Balian,2 Nelson Mores,3 and José Soares Ferreira-Neto2

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo-Escola Paulista de Medicina,1 and Departamento de Medicina Veterinária Preventiva e Saúde Animal, Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo,2 São Paulo, and Empresa Brasileira de Pesquisa Agropecuária, Centro Nacional de Pesquisa em Suínos e Aves, Concórdia-Santa Catarina,3 Brazil

Received 22 February 1999/Returned for modification 8 April 1999/Accepted 27 April 1999

Mycobacterium avium complex (MAC) is composed of environmental mycobacteria found widely in soil, water, and aerosols that can cause disease in animals and humans, especially disseminated infections in AIDS patients. MAC consists of two closely related species, M. avium and M. intracellulare, and may also include other, less-defined groups. The precise differentiation of MAC species is a fundamental step in epidemiological studies and for the evaluation of possible reservoirs for MAC infection in humans and animals. In this study, which included 111 pig and 26 clinical MAC isolates, two novel allelic M. avium PCR-restriction enzyme analysis (PRA) variants were identified, differing from the M. avium PRA prototype in the HaeIII digestion pattern. Mutations in HaeIII sites were confirmed by DNA sequencing. Identification of these isolates as M. avium was confirmed by PCR with DT1-DT6 and IS1245 primers, nucleic acid hybridization with the AccuProbe system, 16S ribosomal DNA sequencing, and biochemical tests. The characterization of M. avium PRA variants can be useful in the elucidation of factors involved in mycobacterial virulence and routes of infection and also has diagnostic significance, since they can be misidentified as M. simiae II and M. kansasii I if the PRA method is used in the clinical laboratory for identification of mycobacteria.

* Corresponding author. Mailing address: Universidade Federal de São Paulo-Escola Paulista de Medicina, Disciplina de Microbiologia, Rua Botucatu, 862 3 andar, 04023-062 São Paulo, Brazil. Phone: (55-11) 5084-3213. Fax: (55-11) 571-6504. E-mail: scleao.dmip{at}epm.br.

Journal of Clinical Microbiology, August 1999, p. 2592-2597, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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