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Journal of Clinical Microbiology, August 1999, p. 2648-2651, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Evaluation of the Granada Agar Plate for Detection of Vaginal and Rectal Group B Streptococci in Pregnant Women

E. García Gil,1 M. C. Rodríguez,2 R. Bartolomé,1 B. Berjano,2 L. Cabero,2 and A. Andreu1,*

Microbiology Service1 and Department of Obstetrics and Gynecology,2 Hospitals Vall d'Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain

Received 24 February 1999/Returned for modification 26 March 1999/Accepted 18 May 1999

Granada medium was evaluated for the detection of group B streptococci (GBS) in vaginal and rectal swabs compared with selective Columbia blood agar and selective Lim broth. From May 1996 to March 1998, 702 pregnant women (35 to 37 weeks of gestation) participated in this three-phase study; 103 (14.7%) of these women carried GBS. In the first phase of the experiment (n = 273 women), vaginorectal specimens were collected on the same swab; the sensitivities of Granada tube, selective Columbia blood agar, and Lim broth were 31.4, 94.3, and 74.3%, respectively. In the second and third phases (n = 429 women), vaginal and rectal specimens were collected separately; the sensitivities of Granada plate, selective Columbia blood agar, and Lim broth (subcultured at 4 h on selective Columbia agar in the second phase and at 18 to 24 h in Granada plate in the third phase) were 91.1, 83.9, and 75%, respectively, in the second phase and 88.5, 90.4, and 63.5%, respectively, in the third phase. There were no statistically significant differences in GBS recovery between the Granada agar plate and selective Columbia blood agar, but the Granada plate provided a clear advantage; the characteristic red-orange colonies produced overnight by GBS can be identified by the naked eye and is so specific that further identification is unnecessary. The use of the Granada tube and Lim broth did not result in increased isolation of GBS. In conclusion, the Granada agar plate is highly sensitive for detecting GBS in vaginal and rectal swabs from pregnant women and can provide results in 18 to 24 h.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospitals Vall d'Hebron, Pg. Vall d'Hebron 119-129, 08035 Barcelona, Spain. Phone: 34-93-274 6894. Fax: 34-93-274 6803. E-mail: anando{at}cs.vhebron.es.

Journal of Clinical Microbiology, August 1999, p. 2648-2651, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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