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Journal of Clinical Microbiology, August 1999, p. 2663-2666, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Mutations in the rpoB Gene of
Rifampin-Resistant Mycobacterium tuberculosis Strains
Isolated Mostly in Asian Countries and Their Rapid Detection by
Line Probe Assay
Kazue
Hirano,
Chiyoji
Abe,* and
Mitsuyoshi
Takahashi
Research Institute of Tuberculosis, Japan
Anti-Tuberculosis Association, Kiyose-shi, Tokyo 204-0022, Japan
Received 4 February 1999/Returned for modification 26 March
1999/Accepted 24 April 1999
Mutations in the rpoB gene of 90 rifampin-resistant
Mycobacterium tuberculosis isolates mostly from Asian
countries were analyzed. Ten distinct single-nucleotide substitutions
were found among the isolates by automated sequencing. A 3-nucleotide
insertion was found in two isolates, and no mutation was found in five
isolates (5.6%). A reverse hybridization-based line probe assay
(INNO-LiPA Rif TB) for rapid detection of the mutations was evaluated
with these isolates. Concordance rates with sequencing results for five
wild-type probes (S probes) and four probes for specific mutations (R
probes) were 96.7 and 100%, respectively. The overall concordance rate
with the in vitro susceptibility testing results was 92.2% (83 of 90 isolates). These results indicate that a commercial line probe assay
kit may be useful for rapid diagnosis of rifampin-resistant tuberculosis.
*
Corresponding author. Mailing address: Research
Institute of Tuberculosis, Japan Anti-Tuberculosis Association,
Kiyose-shi, Tokyo 204-0022, Japan. Phone: 81-424-93-5773. Fax:
81-424-92-4600.
Journal of Clinical Microbiology, August 1999, p. 2663-2666, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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