Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Strains Isolated Mostly in Asian Countries and Their Rapid Detection by Line Probe Assay

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Journal of Clinical Microbiology, August 1999, p. 2663-2666, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Mutations in the rpoB Gene of Rifampin-Resistant Mycobacterium tuberculosis Strains Isolated Mostly in Asian Countries and Their Rapid Detection by Line Probe Assay

Kazue Hirano, Chiyoji Abe,^* and Mitsuyoshi Takahashi

Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose-shi, Tokyo 204-0022, Japan

Received 4 February 1999/Returned for modification 26 March 1999/Accepted 24 April 1999

Mutations in the rpoB gene of 90 rifampin-resistant Mycobacterium tuberculosis isolates mostly from Asian countries were analyzed.Ten distinct single-nucleotide substitutions were found amongthe isolates by automated sequencing. A 3-nucleotide insertionwas found in two isolates, and no mutation was found in five isolates(5.6%). A reverse hybridization-based line probe assay (INNO-LiPARif TB) for rapid detection of the mutations was evaluated withthese isolates. Concordance rates with sequencing results forfive wild-type probes (S probes) and four probes for specificmutations (R probes) were 96.7 and 100%, respectively. The overallconcordance rate with the in vitro susceptibility testing resultswas 92.2% (83 of 90 isolates). These results indicate that a commercialline probe assay kit may be useful for rapid diagnosis of rifampin-resistanttuberculosis.

^* Corresponding author. Mailing address: Research Institute of Tuberculosis, Japan Anti-Tuberculosis Association, Kiyose-shi,Tokyo 204-0022, Japan. Phone: 81-424-93-5773. Fax: 81-424-92-4600.

Journal of Clinical Microbiology, August 1999, p. 2663-2666, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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