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Journal of Clinical Microbiology, August 1999, p. 2674-2677, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Use of Granada Medium To Detect Group B Streptococcal Colonization in Pregnant Women

Manuel Rosa-Fraile,1,* Javier Rodriguez-Granger,1 Marina Cueto-Lopez,1 Antonio Sampedro,1 Enrique Biel Gaye,2 José Manuel Haro,2 and Antonia Andreu3

Microbiology Service1 and Obstetric & Gynecology Department,2 Virgen de las Nieves Hospital, 18014 Granada, and Microbiology Service, Vall D'Hebron Hospital, 08035 Barcelona,3 Spain

Received 4 March 1999/Returned for modification 12 April 1999/Accepted 29 April 1999

Direct inoculation onto Granada medium (GM) in plates and tubes was compared to inoculation into a selective Todd-Hewitt broth (with 8 µg of gentamicin per ml and 15 µg of nalidixic acid per ml) for detection of group B streptococci (GBS) in pregnant women with 800 vaginal and 450 vaginoanorectal samples. Comparatively, GM was found to be as sensitive as the selective broth for the detection of GBS in vaginal specimens and more sensitive than selective broth for the detection of GBS in vaginoanorectal samples (96 versus 82%). The use of GM improved the time to reporting of a GBS-positive result by at least 24 h and reduced the direct cost of screening. We have also found that the inconvenience of anaerobic incubation of GM plates can be avoided when a cover slide is placed upon the inoculum, because aerobic incubation in GM plates with cover slides causes GBS to develop the same pigmentation that it develops with incubation under anaerobic conditions. These data support the routine use of GM plates or tubes as a more accurate, easier, and cheaper method of identification of GBS-colonized women compared to the enrichment broth technique.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Virgen de las Nieves, 18014 Granada, Spain. Phone: 34-958-241109. Fax: 34-958-241282 or 34-958-241245. E-mail: delarosa{at}cica.es.

Journal of Clinical Microbiology, August 1999, p. 2674-2677, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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