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Journal of Clinical Microbiology, August 1999, p. 2709-2714, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Novel Immunoblot Assay Using Four Recombinant Antigens for Diagnosis of Epstein-Barr Virus Primary Infection and Reactivation

M. Buisson,1,* B. Fleurent,2 M. Mak,3 P. Morand,1 L. Chan,4 A. Ng,3 M. Guan,3 D. Chin,3 and J. M. Seigneurin1

Laboratoire de Virologie Médicale Moléculaire, RHAP-CNRS, Faculté de Médecine, 38043 Grenoble, France1; Genelabs Diagnostics S.A., Geneva, Switzerland2; and Genelabs Diagnostics Pte. Ltd.,3 and Bioprocessing Technology Center, National University of Singapore,4 Singapore, Republic of Singapore

Received 28 September 1998/Returned for modification 20 January 1999/Accepted 22 April 1999

A new immunoblot assay, composed of four Epstein-Barr virus (EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23, early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with an immunofluorescence assay on a total of 291 sera. The test was accurate in 94.5% of cases of primary EBV infection, while an immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.

* Corresponding author. Mailing address: Laboratoire de Virologie, CHU/Faculté de médecine, BP 217, 38043 Grenoble, France. Phone: 334 76 76 56 04. Fax: 334 76 76 52 28. E-mail: Marlyse.Buisson{at}ujf-grenoble.fr.

Journal of Clinical Microbiology, August 1999, p. 2709-2714, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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