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Journal of Clinical Microbiology, August 1999, p. 2709-2714, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
Novel Immunoblot Assay Using Four Recombinant
Antigens for Diagnosis of Epstein-Barr Virus Primary Infection
and Reactivation
M.
Buisson,1,*
B.
Fleurent,2
M.
Mak,3
P.
Morand,1
L.
Chan,4
A.
Ng,3
M.
Guan,3
D.
Chin,3 and
J. M.
Seigneurin1
Laboratoire de Virologie Médicale
Moléculaire, RHAP-CNRS, Faculté de Médecine, 38043 Grenoble, France1; Genelabs Diagnostics
S.A., Geneva, Switzerland2; and Genelabs
Diagnostics Pte. Ltd.,3 and
Bioprocessing Technology Center, National University of
Singapore,4 Singapore, Republic of Singapore
Received 28 September 1998/Returned for modification 20 January
1999/Accepted 22 April 1999
A new immunoblot assay, composed of four Epstein-Barr virus
(EBV)-encoded recombinant proteins (virus capsid antigen [VCA] p23,
early antigen [EA] p138, EA p54, and EBNA-1 p72), was compared with
an immunofluorescence assay on a total of 291 sera. The test was
accurate in 94.5% of cases of primary EBV infection, while an
immunoglobulin G anti-VCA p23 band with strong intensity correlated with reactivation.
*
Corresponding author. Mailing address: Laboratoire de
Virologie, CHU/Faculté de médecine, BP 217, 38043 Grenoble,
France. Phone: 334 76 76 56 04. Fax: 334 76 76 52 28. E-mail:
Marlyse.Buisson{at}ujf-grenoble.fr.
Journal of Clinical Microbiology, August 1999, p. 2709-2714, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.
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