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Journal of Clinical Microbiology, August 1999, p. 2717-2718, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Typing of Clinical Herpes Simplex Virus Type 1 and Type 2 Isolates with Monoclonal Antibodies

Jan-Åke Liljeqvist,* Bo Svennerholm, and Tomas Bergström

Department of Virology, University of Göteborg, S-413 46 Göteborg, Sweden

Received 17 December 1998/Returned for modification 7 February 1999/Accepted 21 April 1999

The purpose of this study was to evaluate the performance of a herpes simplex virus (HSV) type 1-specific anti-glycoprotein C-1 monoclonal antibody (MAb) and a type 2-specific anti-glycoprotein G-2 MAb for typing of 2,400 clinical HSV-1 isolates and 2,400 clinical HSV-2 isolates, respectively, using an enzyme immunoassay. The anti-HSV-1 MAb showed sensitivity and specificity of 100%, and the anti-HSV-2 MAb showed a sensitivity of 99.46% and 100% specificity, indicating that these MAbs are suitable for typing of clinical HSV isolates.

* Corresponding author. Mailing address: Department of Virology, University of Göteborg, Guldhedsgatan 10 B, S-413 46 Göteborg, Sweden. Phone: 46 31 3424657. Fax: 46 31 3424960. E-mail: jan-ake.liljeqvist{at}mednet.gu.se.

Journal of Clinical Microbiology, August 1999, p. 2717-2718, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 1999 by the American Society for Microbiology. All rights reserved.