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Journal of Clinical Microbiology, August 1999, p. 2723-2725, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Sequencing of the Ribosomal Intergenic Spacer Region for Strain Identification of Porphyromonas gingivalis

Robert W. Rumpf,1 Ann L. Griffen,2,* Bo-Gui Wen,1,dagger and Eugene J. Leys1

Department of Oral Biology1 and Department of Pediatric Dentistry,2 College of Dentistry, The Ohio State University, Columbus, Ohio 43210

Received 13 January 1999/Returned for modification 30 March 1999/Accepted 20 April 1999

The ribosomal intergenic spacer regions (ISRs) of 19 laboratory strains and 30 clinical samples of Porphyromonas gingivalis were amplified by PCR and sequenced to provide a strain identifier. The ISR is a variable region of DNA located between the conserved 16S and 23S rRNA genes. This makes it an ideal locus for differentiation of strains within a species: primers specific for the conserved flanking genes were used to amplify the ISR, which was then sequenced to identify the strain. We have constructed a P. gingivalis ISR sequence database to facilitate strain identification. ISR sequence analysis provides a strain identifier that can be easily reproduced among laboratories and catalogued for unambiguous comparison.

* Corresponding author. Mailing address: Department of Pediatric Dentistry, College of Dentistry, The Ohio State University, 305 W. 12th Ave., Columbus, OH 43210. Phone: (614) 292-1150. Fax: (614) 688-3077. E-mail: griffen.1{at}osu.edu.

dagger Present address: Department of Biochemistry, Shantou University Medical College, Guangdong Shantou, 515031, People's Republic of China.

Journal of Clinical Microbiology, August 1999, p. 2723-2725, Vol. 37, No. 8
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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