Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

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Journal of Clinical Microbiology, September 1999, p. 2789-2792, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Rapid Slide Latex Agglutination Test for Detection of Methicillin Resistance in Staphylococcus aureus

Arjanne van Griethuysen,¹^,^* Miranda Pouw,² Nan van Leeuwen,² Max Heck,² Piet Willemse,³ Anton Buiting,¹ and Jan Kluytmans³

Department of Clinical Microbiology, St. Elisabeth Hospital, Tilburg,¹ National Institute of Public Health and Environmental Protection, Bilthoven,² and Department of Clinical Microbiology, St. Ignatius Hospital, Breda,³ The Netherlands

Received 8 March 1999/Returned for modification 13 April 1999/Accepted 20 May 1999

The MRSA screen test (Denka Seiken Co., Ltd.), a commercially available, rapid (20-min) slide latex agglutination test forthe determination of methicillin resistance by detection of PBP2a in Staphylococcus aureus, was compared with the oxacillin agarscreen test and PCR detection of the mecA gene. A total of 563S. aureus isolates were tested. Two hundred ninety-six of theisolates were methicillin-susceptible isolates from cultures ofblood from consecutive patients. Also, 267 methicillin-resistantisolates that comprised 248 different phage types were tested.Methicillin resistance was defined as the presence of the mecAgene. Of the 267 mecA gene-positive isolates, 263 were positiveby the MRSA screen test (sensitivity, 98.5%), and all the mecA-genenegative strains were negative by the MRSA screen test (specificity,100%). The oxacillin agar screen test detected methicillin resistancein 250 of the mecA gene-positive isolates (sensitivity, 93.6%).The sensitivity of the MRSA screen test was statistically significantlyhigher than the sensitivity of the oxacillin agar screen test(P < 0.05). The MRSA screen test is a highly sensitive and specifictest for the detection of methicillin resistance. Also, it offersresults within half an hour and is easy to perform, which makesthis test a valuable tool in the ongoing battle against methicillin-resistantS. aureus.

^* Corresponding author. Mailing address: Department of Clinical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 ASTilburg, The Netherlands. Phone: (31) 13-5392650. Fax: (31) 13-5441264.E-mail: a.vgriethuysen{at}worldonline.nl.

Journal of Clinical Microbiology, September 1999, p. 2789-2792, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

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