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Journal of Clinical Microbiology, September 1999, p. 2983-2986, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Immunoglobulin M Antibody Test To Detect Genogroup II Norwalk-Like Virus Infection

James P. Brinker,1 Neil R. Blacklow,1 Xi Jiang,2 Mary K. Estes,3 Christine L. Moe,4 and John E. Herrmann1,*

Division of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts 016551; Center for Pediatric Research, Children's Hospital of the King's Daughters, Eastern Virginia Medical School, Norfolk, Virginia 235102; Division of Molecular Virology, Baylor College of Medicine, Houston, Texas 770303; and Department of Epidemiology, University of North Carolina, Chapel Hill, North Carolina 275994

Received 2 March 1999/Returned for modification 10 May 1999/Accepted 10 June 1999

Sera obtained from adult volunteers inoculated with genogroup II Norwalk-like viruses (NLVs), Hawaii virus, and Snow Mountain virus and from patients involved in outbreaks of gastroenteritis were tested for genogroup II NLV Mexico virus-specific immunoglobulin M (IgM) by use of a monoclonal antibody, recombinant Mexico virus antigen (rMXV)-based IgM capture enzyme-linked immunosorbent assay (ELISA). Sera from genogroup I Norwalk virus (NV)-inoculated volunteers and from patients involved in a genogroup I NLV outbreak were also tested. In sera from those infected with genogroup I NV or NLVs in volunteer and outbreak studies, only 3 of 25 were rMXV IgM positive; in contrast, 24 of 25 were IgM positive for recombinant NV (rNV). In sera from those infected with genogroup II NLVs in volunteer and outbreak studies, 28 of 47 were rMXV IgM positive and none were IgM positive for rNV, showing the specificity of each IgM test for its respective genogroup. In an outbreak of gastroenteritis not characterized as being of viral etiology but suspected to be due to NV, 7 of 13 persons had IgM responses to rMXV, whereas none had IgM responses to rNV, thus establishing the diagnosis as genogroup II NLV infection. The rMXV-based IgM capture ELISA developed is specific for the diagnosis of genogroup II NLV infections.

* Corresponding author. Mailing address: Division of Infectious Diseases, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655. Phone: (508) 856-2155. Fax: (508) 856-5981. E-mail: John.E.Herrmann{at}banyan.ummed.edu.

Journal of Clinical Microbiology, September 1999, p. 2983-2986, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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