This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Leke, R. F. G.
Right arrow Articles by Taylor, D. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leke, R. F. G.
Right arrow Articles by Taylor, D. W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 1999, p. 2992-2996, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of the Plasmodium falciparum Antigen Histidine-Rich Protein 2 in Blood of Pregnant Women: Implications for Diagnosing Placental Malaria

Rose F. G. Leke,1 Rosine R. Djokam,1 Robinson Mbu,1 Robert J. Leke,1 Josephine Fogako,1 Rosette Megnekou,1 Simon Metenou,1 Grace Sama,1 Yuan Zhou,2 Timothy Cadigan,2 Marcela Parra,2 and Diane Wallace Taylor2,*

Faculty of Medicine and Biomedical Sciences, The Biotechnology Center, University of Yaounde 1, Yaounde, Cameroon,1 and Department of Biology, Georgetown University, Washington, D.C. 200572

Received 9 February 1999/Returned for modification 7 April 1999/Accepted 7 June 1999

Pregnant women have an increased susceptibility to infection by Plasmodium falciparum. Parasites may be present in the placenta yet not detectable in peripheral blood smears by routine light microscopy. In order to determine how frequently misdiagnosis occurs, peripheral blood and placental samples were collected from 1,077 Cameroonian women at the time of giving birth and examined for the presence of malarial parasites by using light microscopy. Results showed that 20.1% of the women who had placental malaria were peripheral blood smear negative. Thus, malarial infection was not detected by microscopic examination of peripheral blood smears from approximately one out of five malaria-infected women. Since P. falciparum parasites secrete histidine-rich protein 2 (HRP-2), we sought to determine if detecting HRP-2 in either peripheral plasma or whole blood might be used to diagnose the presence of parasites "hidden" in the placenta. Samples of peripheral plasma from 127 women with different levels of placental malarial infection were assayed by HRP-2-specific enzyme-linked immunosorbent assay. HRP-2 was detected in 88% of the women with placental malaria who tested negative by blood smear. Additionally, whole blood was obtained from 181 women and tested for HRP-2 with a rapid, chromatographic strip test (ICT). The ICT test accurately detected malarial infection in 89.1% of P. falciparum-infected women. Furthermore, 94% of women with malaria were accurately diagnosed by using a combination of microscopy and the ICT test. Thus, detection of HRP-2 in conjunction with microscopy should improve diagnosis of malaria in pregnant women.

* Corresponding author. Mailing address: Department of Biology, Reiss Science Center, Rm. 334, Georgetown University, 37th and O St., NW, Washington, DC 20057. Phone: (202) 687-5972. Fax: (202) 687-5662. E-mail: taylordw{at}gusun.georgetown.edu.

Journal of Clinical Microbiology, September 1999, p. 2992-2996, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Yatich, N. J., Yi, J., Agbenyega, T., Turpin, A., Rayner, J. C., Stiles, J. K., Ellis, W. O., Funkhouser, E., Ehiri, J. E., Williams, J. H., Jolly, P. E. (2009). Malaria and Intestinal Helminth Co-infection Among Pregnant Women in Ghana: Prevalence and Risk Factors. Am J Trop Med Hyg 80: 896-901 [Abstract] [Full Text]  
  • Martin, S. K., Rajasekariah, G-H., Awinda, G., Waitumbi, J., Kifude, C. (2009). Unified Parasite Lactate Dehydrogenase and Histidine-Rich Protein ELISA for Quantification of Plasmodium falciparum. Am J Trop Med Hyg 80: 516-522 [Abstract] [Full Text]  
  • Sharma, M. K., Rao, V. K., Agarwal, G. S., Rai, G. P., Gopalan, N., Prakash, S., Sharma, S. K., Vijayaraghavan, R. (2008). Highly Sensitive Amperometric Immunosensor for Detection of Plasmodium falciparum Histidine-Rich Protein 2 in Serum of Humans with Malaria: Comparison with a Commercial Kit. J. Clin. Microbiol. 46: 3759-3765 [Abstract] [Full Text]  
  • Kifude, C. M., Rajasekariah, H. G., Sullivan, D. J. Jr., Stewart, V. A., Angov, E., Martin, S. K., Diggs, C. L., Waitumbi, J. N. (2008). Enzyme-Linked Immunosorbent Assay for Detection of Plasmodium falciparum Histidine-Rich Protein 2 in Blood, Plasma, and Serum. CVI 15: 1012-1018 [Abstract] [Full Text]  
  • Murray, C. K., Gasser, R. A. Jr., Magill, A. J., Miller, R. S. (2008). Update on Rapid Diagnostic Testing for Malaria. Clin. Microbiol. Rev. 21: 97-110 [Abstract] [Full Text]  
  • Wongsrichanalai, C., Barcus, M. J., Muth, S., Sutamihardja, A., Wernsdorfer, W. H. (2007). A Review of Malaria Diagnostic Tools: Microscopy and Rapid Diagnostic Test (RDT). Am J Trop Med Hyg 77: 119-127 [Abstract] [Full Text]  
  • COTTRELL, G., MARY, J.-Y., BARRO, D., COT, M. (2005). IS MALARIAL PLACENTAL INFECTION RELATED TO PERIPHERAL INFECTION AT ANY TIME OF PREGNANCY?. Am J Trop Med Hyg 73: 1112-1118 [Abstract] [Full Text]  
  • SINGH, N., SAXENA, A., AWADHIA, S. B., SHRIVASTAVA, R., SINGH, M. P. (2005). EVALUATION OF A RAPID DIAGNOSTIC TEST FOR ASSESSING THE BURDEN OF MALARIA AT DELIVERY IN INDIA. Am J Trop Med Hyg 73: 855-858 [Abstract] [Full Text]  
  • NYUNT, M., PISCIOTTA, J., FELDMAN, A. B., THUMA, P., SCHOLL, P. F., DEMIREV, P. A., LIN, J. S., SHI, L., KUMAR, N., SULLIVAN, D. J. JR. (2005). DETECTION OF PLASMODIUM FALCIPARUM IN PREGNANCY BY LASER DESORPTION MASS SPECTROMETRY. Am J Trop Med Hyg 73: 485-490 [Abstract] [Full Text]  
  • Malhotra, I., Dent, A., Mungai, P., Muchiri, E., King, C. L. (2005). Real-Time Quantitative PCR for Determining the Burden of Plasmodium falciparum Parasites during Pregnancy and Infancy. J. Clin. Microbiol. 43: 3630-3635 [Abstract] [Full Text]  
  • BELL, D. R., WILSON, D. W., MARTIN, L. B. (2005). FALSE-POSITIVE RESULTS OF A PLASMODIUM FALCIPARUM HISTIDINE-RICH PROTEIN 2-DETECTING MALARIA RAPID DIAGNOSTIC TEST DUE TO HIGH SENSITIVITY IN A COMMUNITY WITH FLUCTUATING LOW PARASITE DENSITY. Am J Trop Med Hyg 73: 199-203 [Abstract] [Full Text]  
  • Malhotra, I., Mungai, P., Muchiri, E., Ouma, J., Sharma, S., Kazura, J. W., King, C. L. (2005). Distinct Th1- and Th2-Type Prenatal Cytokine Responses to Plasmodium falciparum Erythrocyte Invasion Ligands. Infect. Immun. 73: 3462-3470 [Abstract] [Full Text]  
  • TAKO, E. A., ZHOU, A., LOHOUE, J., LEKE, R., TAYLOR, D. W., LEKE, R. F. G. (2005). RISK FACTORS FOR PLACENTAL MALARIA AND ITS EFFECT ON PREGNANCY OUTCOME IN YAOUNDE, CAMEROON. Am J Trop Med Hyg 72: 236-242 [Abstract] [Full Text]  
  • TER KUILE, F. O., PARISE, M. E., VERHOEFF, F. H., UDHAYAKUMAR, V., NEWMAN, R. D., VAN EIJK, A. M., ROGERSON, S. J., STEKETEE, R. W. (2004). THE BURDEN OF CO-INFECTION WITH HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 AND MALARIA IN PREGNANT WOMEN IN SUB-SAHARAN AFRICA. Am J Trop Med Hyg 71: 41-54 [Abstract] [Full Text]  
  • SINGER, L. M., NEWMAN, R. D., DIARRA, A., MORAN, A. C., HUBER, C. S., STENNIES, G., SIRIMA, S. B., KONATE, A., YAMEOGO, M., SAWADOGO, R., BARNWELL, J. W., PARISE, M. E. (2004). EVALUATION OF A MALARIA RAPID DIAGNOSTIC TEST FOR ASSESSING THE BURDEN OF MALARIA DURING PREGNANCY. Am J Trop Med Hyg 70: 481-485 [Abstract] [Full Text]  
  • SUGUITAN, A. L. JR., CADIGAN, T. J., NGUYEN, T. A., ZHOU, A., LEKE, R. J. I., METENOU, S., THUITA, L., MEGNEKOU, R., FOGAKO, J., LEKE, R. G. F., TAYLOR, D. W. (2003). MALARIA-ASSOCIATED CYTOKINE CHANGES IN THE PLACENTA OF WOMEN WITH PRE-TERM DELIVERIES IN YAOUNDE, CAMEROON. Am J Trop Med Hyg 69: 574-581 [Abstract] [Full Text]  
  • Rogerson, S. J., Mkundika, P., Kanjala, M. K. (2003). Diagnosis of Plasmodium falciparum Malaria at Delivery: Comparison of Blood Film Preparation Methods and of Blood Films with Histology. J. Clin. Microbiol. 41: 1370-1374 [Abstract] [Full Text]  
  • TER KUILE, F. O., TERLOUW, D. J., PHILLIPS-HOWARD, P. A., HAWLEY, W. A., FRIEDMAN, J. F., KARIUKI, S. K., SHI, Y. P., KOLCZAK, M. S., LAL, A. A., VULULE, J. M., NAHLEN, B. L. (2003). REDUCTION OF MALARIA DURING PREGNANCY BY PERMETHRIN-TREATED BED NETS IN AN AREA OF INTENSE PERENNIAL MALARIA TRANSMISSION IN WESTERN KENYA. Am J Trop Med Hyg 68: 50-60 [Abstract] [Full Text]  
  • Ofori, M. F., Staalsoe, T., Bam, V., Lundquist, M., David, K. P., Browne, E. N. L., Akanmori, B. D., Hviid, L. (2003). Expression of Variant Surface Antigens by Plasmodium falciparum Parasites in the Peripheral Blood of Clinically Immune Pregnant Women Indicates Ongoing Placental Infection. Infect. Immun. 71: 1584-1586 [Abstract] [Full Text]  
  • Mankhambo, L., Kanjala, M., Rudman, S., Lema, V. M., Rogerson, S. J. (2002). Evaluation of the OptiMAL Rapid Antigen Test and Species-Specific PCR To Detect Placental Plasmodium falciparum Infection at Delivery. J. Clin. Microbiol. 40: 155-158 [Abstract] [Full Text]  
  • Mockenhaupt, F. P., Ulmen, U., von Gaertner, C., Bedu-Addo, G., Bienzle, U. (2002). Diagnosis of Placental Malaria. J. Clin. Microbiol. 40: 306-308 [Abstract] [Full Text]  
  • Forney, J. R., Magill, A. J., Wongsrichanalai, C., Sirichaisinthop, J., Bautista, C. T., Heppner, D. G., Miller, R. S., Ockenhouse, C. F., Gubanov, A., Shafer, R., DeWitt, C. C., Quino-Ascurra, H. A., Kester, K. E., Kain, K. C., Walsh, D. S., Ballou, W. R., Gasser, R. A. Jr. (2001). Malaria Rapid Diagnostic Devices: Performance Characteristics of the ParaSight F Device Determined in a Multisite Field Study. J. Clin. Microbiol. 39: 2884-2890 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»