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Journal of Clinical Microbiology, September 1999, p. 3022-3024, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Discrimination of Multidrug-Resistant Mycobacterium tuberculosis IS6110 Fingerprint Subclusters by rpoB Gene Mutation Analysis

Isabel Portugal,* Sara Maia, and José Moniz-Pereira

Unidade dos Retrovírus e Infecções Associadas and Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, 1649-019 Lisbon, Portugal

Received 25 January 1999/Returned for modification 20 March 1999/Accepted 18 May 1999

The rpoB gene mutations in a 69-bp region of the gene, resulting in resistance to rifampin, were used to discriminate between Mycobacterium tuberculosis IS6110 fingerprint subclusters. These subclusters exhibited identical IS6110 fragments or had one or two additional fragments. In the two major subclusters all the analyzed strains have the same variant rpoB allele but are different from each other, suggesting the occurrence of independent outbreaks.

* Corresponding author. Mailing address: Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, Av. Forças Armadas, 1649-019 Lisbon, Portugal. Phone: 351-1-7946439. Fax: 351-1-7934212. E-mail: isabel.portugal{at}ff.ul.pt.

Journal of Clinical Microbiology, September 1999, p. 3022-3024, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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