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Journal of Clinical Microbiology, September 1999, p. 3034-3036, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Analysis by Enzyme-Linked Immunosorbent Assay and PCR of Human Liver Abscess Aspirates from Patients in China for Entamoeba histolytica

G. Zengzhu,1,2 R. Bracha,1 Y. Nuchamowitz,1 W. Cheng-I,2 and D. Mirelman1,*

Department of Biological Chemistry, Weizmann Institute of Science, Rehovot, Israel,1 and Beijing Tropical Medicine Research Institute, Beijing, People's Republic of China2

Received 23 October 1998/Returned for modification 8 December 1998/Accepted 25 May 1999

Aspirates of liver abscesses were analyzed for Entamoeba histolytica. PCR detected a gene encoding a 30-kDa protein in all samples but detected the ribosomal DNA gene in only 14 (33.3%) samples. Enzyme-linked immunosorbent assay detected antigen in 41 (97.6%) samples. PCR analysis of a strain-specific antigen (SSG) revealed that abscesses were caused by various strains.

* Corresponding author. Mailing address: Department of Biological Chemistry, Weizmann Institute of Science, Rehovot 76100, Israel. Phone: 972-8-9343160. Fax: 972-8-9468256. E-mail: bfmirelm{at}weizmann.weizmann.ac.il.

Journal of Clinical Microbiology, September 1999, p. 3034-3036, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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