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Journal of Clinical Microbiology, September 1999, p. 3072-3073, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.

Detection of Chlamydia trachomatis Endocervical Infections by Ligase Chain Reaction versus ACCESS Chlamydia Antigen Assay

K. B. Waites,1,* K. R. Smith,2 M. A. Crum,3 R. D. Hockett,1 A. H. Wells,1 and E. W. Hook III2

Departments of Pathology,1 Medicine,2 and Hospital Laboratories,3 University of Alabama at Birmingham, Birmingham, Alabama

Received 16 February 1999/Returned for modification 8 April 1999/Accepted 28 May 1999

Ligase chain reaction (LCR) was compared with ACCESS immunoassay for detection of chlamydial infections in females. Despite efforts to improve ACCESS performance by evaluation of specimens that were in the test performance "grey zone," LCR remained more sensitive and was less expensive to perform. ACCESS had a sensitivity of 83.9%, a specificity of 99.7%, a positive predictive value of 96.3%, and a negative predictive value of 98.5%.

* Corresponding author. Mailing address: Department of Pathology WP 230, University of Alabama at Birmingham, 618 South 18th St., Birmingham, AL 35233-7331. Phone: (205) 934-6421. Fax: (205) 975-4468. E-mail: waites{at}path.uab.edu.

Journal of Clinical Microbiology, September 1999, p. 3072-3073, Vol. 37, No. 9
0095-1137/99/$04.00+0
Copyright © 1999, American Society for Microbiology. All rights reserved.


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Copyright © 1999 by the American Society for Microbiology. All rights reserved.