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Journal of Clinical Microbiology, January 2000, p. 120-124, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Isolation, Cultivation, and Characterization of Borrelia burgdorferi from Rodents and Ticks in the Charleston Area of South Carolina

J. H. Oliver Jr.,1,* K. L. Clark,2 F. W. Chandler Jr.,3 L. Tao,1 A. M. James,1,dagger C. W. Banks,1 L. O. Huey,3 A. R. Banks,1 D. C. Williams,4 and L. A. Durden1

Institute of Arthropodology and Parasitology, Department of Biology, Georgia Southern University, Statesboro, Georgia 30460-80561; Department of Health Science, University of North Florida, Jacksonville, Florida 322242; Department of Pathology, Medical College of Georgia, Augusta, Georgia 30912-36053; and Cypress Gardens, Moncks Corner, South Carolina 294614

Received 6 July 1999/Returned for modification 24 August 1999/Accepted 28 September 1999

Twenty-eight Borrelia burgdorferi isolates from the Charleston, S.C., area are described. This represents the first report and characterization of the Lyme disease spirochete from that state. The isolates were obtained from December 1994 through December 1995 from the tick Ixodes scapularis, collected from vegetation, and from the rodents Peromyscus gossypinus (cotton mouse), Neotoma floridana (eastern wood rat), and Sigmodon hispidus (cotton rat). All isolates were screened immunologically by indirect immunofluorescence with monoclonal antibodies to B. burgdorferi-specific outer surface protein A (OspA) (antibodies H5332 and H3TS) and B. burgdorferi-specific OspB (antibodies H6831 and H614), a Borrelia (genus)-specific antiflagellin antibody (H9724), Borrelia hermsii-specific antibodies (H9826 and H4825), and two polyclonal antibodies (one to Borrelia species and another to B. burgdorferi). Six of the isolates were analyzed by exposing Western blots to monoclonal antibodies H5332, H3TS, H6831, and H9724. All isolates were also analyzed by PCR with five pairs of primers known to amplify selected DNA target sequences specifically reported to be present in the reference strain, B. burgdorferi B-31. The protein profiles of six of the isolates (two from ticks, one from a cotton mouse, two from wood rats, and one from a cotton rat) also were compared by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We conclude that the 28 Charleston isolates are B. burgdorferi sensu stricto based on their similarities to the B. burgdorferi B-31 reference strain.

* Corresponding author. Mailing address: Institute of Arthropodology and Parasitology, P.O. Box 8056, Georgia Southern University, Statesboro, GA 30460-8056. Phone: (912) 681-5564. Fax: (912) 681-0559. E-mail: JOliver{at}GaSou.edu.

dagger Present address: CDC (NCID) Div. of Vector-Borne Infectious Diseases, Foothills Campus, Fort Collins, CO 80522.

Journal of Clinical Microbiology, January 2000, p. 120-124, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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