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Journal of Clinical Microbiology, January 2000, p. 185-190, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Phenotypic and Molecular Typing of Nosocomial Methicillin-Resistant Staphylococcus aureus Strains Susceptible to Gentamicin Isolated in France from 1995 to 1997

Jacques-Olivier Galdbart,1,2 Anne Morvan,1 and Nevine El Solh1,*

Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 75724 Paris Cedex 15,1 and Service de Microbiologie, Hôpital Beaujon, A.P.-H.P., 92100 Clichy,2 France

Received 12 May 1999/Returned for modification 4 October 1999/Accepted 28 October 1999

Methicillin-resistant strains susceptible to gentamicin (Gms MRSA) have emerged since 1993 in several French hospitals. To study whether particular clones have spread in various French cities and whether some clones are related to gentamicin-resistant (Gmr) MRSA strains, various methods (antibiotyping, phage typing, determination of SmaI macrorestriction patterns before and after hybridization with IS256 transposase and aacA-aphD probes) were used to compare 62 Gms MRSA strains isolated from 1995 to 1997 in nine cities and 15 Gmr MRSA strains. Eighteen major SmaI genotypes were identified, of which 11 included only Gms MRSA strains and 5 included only Gmr MRSA strains. Each of the Gmr MRSA strains contained 6 to 13 SmaI fragments hybridizing with the insertion sequence IS256, of which a single band also hybridized with the aacA-aphD gene. No such hybridizing sequences were detected in 60 of the 62 Gms MRSA strains. Thus, the divergence between Gmr and Gms MRSA strains is revealed, not only by their distributions in distinct SmaI genotypes but also by the differences in hybridization patterns. Two of the 62 Gms MRSA strains had the uncommon feature of carrying several SmaI bands hybridizing with IS256, suggesting that they are possibly related to the Gmr MRSA strains grouped in the same SmaI genotype. Five of the 11 SmaI genotypes including only Gms MRSA strains contained strains from diverse cities, isolated during different years and with different antibiograms, suggesting that some clones have spread beyond their cities of origin and persisted.

* Corresponding author. Mailing address: Unité des Staphylocoques, National Reference Center for Staphylococci Institut Pasteur, 75724 Paris Cedex 15, France. Phone: (33) 01 45 68 83 63. Fax: (33) 01 40 61 31 63. E-mail: nelsolh{at}pasteur.fr.

Journal of Clinical Microbiology, January 2000, p. 185-190, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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