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Journal of Clinical Microbiology, January 2000, p. 191-197, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Restriction Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis Isolated from Countries in the Western Pacific Region

Young-Kil Park, Gill-Han Bai, and Sang-Jae Kim*

Korean Institute of Tuberculosis, Korean National Tuberculosis Association, Seoul, Korea

Received 19 May 1999/Returned for modification 19 July 1999/Accepted 13 October 1999

A total of 422 Mycobacterium tuberculosis isolates from eight countries were subjected to IS6110 and IS1081 DNA fingerprinting by means of restriction fragment analysis to characterize M. tuberculosis strains from each country. Chinese, Mongolian, Hong Kong, Filipino, and Korean isolates had comparatively more copies of IS6110 (proportion with eight or more copies; 95% ± 5%), while Thai, Malaysian, and Vietnamese isolates had fewer copies (proportion with eight or more copies, 60% ± 4%). We found a number of novel IS1081 types in this study. One IS1081 type was present in 56% of Filipino isolates, had a specific 6.6-kb PvuII fragment in its IS6110 DNA fingerprint, and was termed the "Filipino family." The IS1081 types of Thai isolates had interposing characteristics between the characteristics of northeastern Asian and southeastern Asian IS1081 types. A 1.3-kb single-copy IS6110 fragment was found only in Vietnamese M. tuberculosis isolates. Although M. tuberculosis isolates from each country had comparatively similar characteristics depending on the classification factor, each country's isolates showed characteristic DNA fingerprints and differed slightly from the isolates from the other countries in either the mode number of IS6110 copies or the distribution of IS1081 types.

* Corresponding author. Mailing address: Korean Institute of Tuberculosis/Korean National Tuberculosis Association, 14 Woomyundong, Sochogu, Seoul 137-140, Korea. Phone: (82) 2-576-0357. Fax: (82) 2-573-1914. E-mail: kit{at}soback.kornet21.net.

Journal of Clinical Microbiology, January 2000, p. 191-197, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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