This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Jiang, B.
Right arrow Articles by Lee, C.-H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jiang, B.
Right arrow Articles by Lee, C.-H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2000, p. 241-245, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Typing of Histoplasma capsulatum Isolates Based on Nucleotide Sequence Variation in the Internal Transcribed Spacer Regions of rRNA Genes

Bingdong Jiang,1 Marilyn S. Bartlett,1 Stephen D. Allen,1 James W. Smith,1 L. Joseph Wheat,1,2 Patricia A. Connolly,2 and Chao-Hung Lee1,*

Department of Pathology and Laboratory Medicine1 and Department of Medicine,2 Indiana University School of Medicine, Indianapolis, Indiana 46202

Received 9 April 1999/Returned for modification 14 June 1999/Accepted 28 September 1999

The nucleotide sequences of internal transcribed spacer (ITS) regions of rRNA genes of 24 isolates of Histoplasma capsulatum were examined. The results indicate that the sequences of ITS regions in different isolates are not identical. Sequence variations were found at 20 positions in the 496 bp that were sequenced. Ten different sequence patterns, designated types A through H, were observed when the sequences from the 24 isolates were aligned. Twelve isolates from Indianapolis were classified into four different types. Two isolates from New York belonged to type G. Three isolates from different cities were type F. The remaining six isolates were of different types.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Indiana University School of Medicine, 1120 South Dr., FH 419, Indianapolis, IN 46202. Phone: (317) 274-2596. Fax: (317) 278-0643. E-mail: chlee{at}iupui.edu.

Journal of Clinical Microbiology, January 2000, p. 241-245, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Zarnowski, R., Miyazaki, M., Dobrzyn, A., Ntambi, J. M., Woods, J. P. (2007). Typing of Histoplasma capsulatum strains by fatty acid profile analysis. J Med Microbiol 56: 788-797 [Abstract] [Full Text]  
  • Tseng, T.-C., Liaw, S.-J., Hsiao, C.-H., Wang, C.-Y., Lee, L.-N., Huang, T.-S., Hsueh, P.-R. (2005). Molecular Evidence of Recurrent Histoplasmosis with 9-Year Latency in a Patient with Addison's Disease. J. Clin. Microbiol. 43: 4911-4913 [Abstract] [Full Text]  
  • Hebeler-Barbosa, F., Morais, F. V., Montenegro, M. R., Kuramae, E. E., Montes, B., McEwen, J. G., Bagagli, E., Puccia, R. (2003). Comparison of the Sequences of the Internal Transcribed Spacer Regions and PbGP43 Genes of Paracoccidioides brasiliensis from Patients and Armadillos (Dasypus novemcinctus). J. Clin. Microbiol. 41: 5735-5737 [Abstract] [Full Text]  
  • Spencer, W. H., Chan, C.-C., Shen, D. F., Rao, N. A. (2003). Detection of Histoplasma capsulatum DNA in Lesions of Chronic Ocular Histoplasmosis Syndrome. Arch Ophthalmol 121: 1551-1555 [Abstract] [Full Text]  
  • Martagon-Villamil, J., Shrestha, N., Sholtis, M., Isada, C. M., Hall, G. S., Bryne, T., Lodge, B. A., Reller, L. B., Procop, G. W. (2003). Identification of Histoplasmacapsulatum from Culture Extracts by Real-Time PCR. J. Clin. Microbiol. 41: 1295-1298 [Abstract] [Full Text]  
  • Muniz, M. d. M., Pizzini, C. V., Peralta, J. M., Reiss, E., Zancope-Oliveira, R. M. (2001). Genetic Diversity of Histoplasma capsulatum Strains Isolated from Soil, Animals, and Clinical Specimens in Rio de Janeiro State, Brazil, by a PCR-Based Random Amplified Polymorphic DNA Assay. J. Clin. Microbiol. 39: 4487-4494 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»