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Journal of Clinical Microbiology, January 2000, p. 292-300, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Methods for the Epidemiological Typing of Salmonella enterica Serotype Typhi from Hong Kong and Vietnam

J. M. Ling,1,* N. W. S. Lo,1 Y. M. Ho,2 K. M. Kam,3 Nguyen Thi Tuyet Hoa,4 Le Thi Phi,4 and A. F. Cheng1

Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, Shatin, New Territories,1 Department of Microbiology, The University of Hong Kong, Queen Mary Hospital,2 and Institute of Pathology, Sai Ying Pun Hospital, Sai Ying Pun,3 Hong Kong SAR, China, and Department of Microbiology, Centre for Tropical Diseases, Cho Quan Hospital, Ho Chi Minh City, Vietnam4

Received 26 July 1999/Returned for modification 28 September 1999/Accepted 8 October 1999

A total of 217 and 73 strains of Salmonella enterica serotype Typhi isolated from 1985 to 1997 in Hong Kong and in 2 months of 1989 and 1990 in Vietnam, respectively, were studied. These isolates were typed by plasmid profile analysis, plasmid fingerprinting, ribotyping with PstI, and total DNA fingerprinting with NarI. There appeared to be no major outbreak of typhoid fever in Hong Kong during the study period since there was considerable heterogeneity among the isolates. Isolates from Hong Kong were different from those from Vietnam. Thirty-seven percent of Vietnamese isolates belonged to two predominant clones, with the rest being heterogeneous in nature. Total DNA fingerprinting supplemented with ribotyping could be a reliable and rapid method for epidemiological typing of S. enterica serotype Typhi.

* Corresponding author. Mailing address: Department of Microbiology, The Chinese University of Hong Kong, The Prince of Wales Hospital, 30-32 Ngan Shing St., Shatin, New Territories, Hong Kong SAR, China. Phone: (852) 2632 3333. Fax: (852) 2647 3227. E-mail: b012765{at}mailserv.cuhk.edu.hk.

Journal of Clinical Microbiology, January 2000, p. 292-300, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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