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Journal of Clinical Microbiology, January 2000, p. 301-308, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Differentiation of Phylogenetically Related Slowly
Growing Mycobacteria by Their gyrB Sequences
Hiroaki
Kasai,1,*
Takayuki
Ezaki,2 and
Shigeaki
Harayama1
Marine Biotechnology Institute, Kamaishi
Laboratories, 3-75-1 Heita, Kamaishi, Iwate
026-0001,1 and Department of
Microbiology, Gifu University School of Medicine, Gifu, Gifu
500-8705,2 Japan
Received 27 May 1999/Returned for modification 18 August
1999/Accepted 4 October 1999
The conventional methods for identifying mycobacterial species are
based on their phenotypic characterization. Since some problematic
species are slow growers, their taxonomy takes several weeks or months
to identify. The ribosomal DNA (rDNA) sequence-based identification
strategy has been adopted to solve this problem. More recently, the
gyrB sequences have been shown to be useful phylogenetic
markers for the identification of species. We determined the
gyrB sequences of 43 slowly growing strains belonging to 15 species in the genus Mycobacterium. The frequencies of base
substitutions in the gyrB sequences were comparable to
those in the 16S-23S rDNA internal transcribed spacer (ITS) sequences.
The ITS sequences of four species belonging to the M. tuberculosis complex (M. tuberculosis, M. bovis, M. africanum, and M. microti) were
100% identical, while four synonymous substitutions were found in the
gyrB sequences of these strains. Based on the differences
found in the gyrB sequences, we developed PCR and
PCR-restriction fragment length polymorphism methods to discriminate
these species.
*
Corresponding author. Mailing address: Marine
Biotechnology Institute, Kamaishi Laboratories, 3-75-1 Heita,
Kamaishi, Iwate 026-0001, Japan. Phone: 81-193-26-6544. Fax:
81-193-26-6584/6592. E-mail:
hiroaki.kasai{at}kamaishi.mbio.co.jp.
Journal of Clinical Microbiology, January 2000, p. 301-308, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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