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Journal of Clinical Microbiology, January 2000, p. 313-317, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Whole-Cell and OspC Enzyme-Linked Immunosorbent Assays for Discrimination of Early Lyme Borreliosis from OspA Vaccination

Chad A. Wieneke,1,2,dagger Steven D. Lovrich,1,* Steven M. Callister,1,3 Dean A. Jobe,1 Jennifer A. Marks,1 and Ronald F. Schell2,4

Microbiology Research Laboratory1 and Section of Infectious Diseases,3 Gundersen Lutheran Medical Center, La Crosse, Wisconsin 54601, and Department of Medical Microbiology and Immunology2 and Wisconsin State Laboratory of Hygiene,4 University of Wisconsin, Madison, Wisconsin 53706

Received 21 May 1999/Returned for modification 23 September 1999/Accepted 14 October 1999

A recombinant Lyme borreliosis vaccine consisting of outer surface protein A (OspA) is commercially available for vaccination of humans against infection with Borrelia burgdorferi. Vaccination with OspA induces an antibody response that makes serologic interpretation of infection with B. burgdorferi difficult, especially by screening tests based on whole-cell preparations of B. burgdorferi. We show that an enzyme-linked immunosorbent assay with B. burgdorferi sensu stricto 50772, which lacks the plasmid encoding OspA and OspB, or a full-length recombinant OspC protein can identify patients infected with B. burgdorferi. We found that 69 and 65% of serum samples from patients with case-defined early Lyme borreliosis had anti-B. burgdorferi sensu stricto 50772 and anti-OspC reactivities, respectively. In addition, little or no reactivity was detected with sera obtained from individuals vaccinated with OspA. Unfortunately, 51 and 33% of sera from healthy patients and sera from patients with other illnesses were also reactive against B. burgdorferi sensu stricto 50772 and OspC, respectively. Although these assays can discriminate B. burgdorferi infection from vaccination with OspA, their lack of specificity highlights the necessity for confirming equivocal or positive reactivities with more specific serodiagnostic tests.

* Corresponding author. Mailing address: Microbiology Research Laboratory, Gundersen Lutheran Medical Center, 1836 South Ave., La Crosse, WI 54601. Phone: (608) 782-7300, ext. 3743. Fax: (608) 791-6602. E-mail: slovrich{at}centuryinter.net.

dagger Present address: R&D Systems, Minneapolis, MN 55413.

Journal of Clinical Microbiology, January 2000, p. 313-317, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

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