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Journal of Clinical Microbiology, January 2000, p. 323-326, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Stability of Plasma Human Immunodeficiency Virus Load in VACUTAINER PPT Plasma Preparation Tubes during Overnight Shipment

Mark Holodniy,1,2 Lynne Rainen,3,* Steve Herman,4 and Belinda Yen-Lieberman5

AIDS Research Center, VA Palo Alto Health Care System,1 and Division of Infectious Diseases and Geographic Medicine, Stanford University,2 Palo Alto, and Roche Molecular Systems, Alameda,4 California; Becton Dickinson Vacutainer Systems, Franklin Lakes, New Jersey3; and Department of Clinical Microbiology, Cleveland Clinic, Cleveland, Ohio5

Received 21 June 1999/Returned for modification 18 August 1999/Accepted 29 September 1999

VACUTAINER PPT plasma preparation tubes were evaluated to determine the effects of various handling and shipping conditions on plasma human immunodeficiency virus (HIV) load determinations. Plasmas obtained from PPT tubes stored and shipped under nine different conditions were compared to conventional EDTA tube plasmas stored at -70°C within 2 h after phlebotomy. Compared to viral loads in frozen EDTA plasma, those in PPT tube plasma that was frozen immediately and either separated or shipped in situ were not significantly different. Viral loads in PPT tube plasma after storage for 6 h at either room temperature or 4°C, followed by shipment at ambient temperature or on wet or dry ice, were not significantly different from baseline viral loads in EDTA or PPT plasma. The results of this study indicate that the HIV load in PPT tube plasma is equivalent to that in standard EDTA plasma. Plasma viral load is not affected by storage or shipment temperature when plasma is collected in PPT tubes. Furthermore, plasmas can be shipped in spun PPT tubes, and the tubes provide a safer and more convenient method for sample collection and transport than regular EDTA tubes.

* Corresponding author. Mailing address: Becton Dickinson VACUTAINER Systems, 1 Becton Dr., M/C338, Franklin Lakes, NJ 07417. Phone: (201) 847-4559. Fax: (201) 847-4851. E-mail: lynne_rainen{at}bdhq.bd.com.

Journal of Clinical Microbiology, January 2000, p. 323-326, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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