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Journal of Clinical Microbiology, January 2000, p. 398-401, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the BACTEC MGIT 960 and the MB/BacT Systems for Recovery of Mycobacteria from Clinical Specimens and for Species Identification by DNA AccuProbe

Fernando Alcaide,1,* Miguel Angel Benítez,1 Josep M. Escribà,2 and Rogelio Martín1

Servei de Microbiologia, Hospital "Princeps d'Espanya," Ciutat Sanitària i Universitària de Bellvitge, 08907 L'Hospitalet de Llobregat, Barcelona, Spain,1 and Cap de Drassanes, Institut Català de la Salut, Barcelona, Spain2

Received 20 April 1999/Returned for modification 26 August 1999/Accepted 15 October 1999

A total of 120 mycobacterial isolates were recovered from 1,068 clinical specimens. Of these, 82.5% were in MGIT 960, 83.3% were in MB/BacT, 80% were in BACTEC 460, and 70% were on Löwenstein-Jensen medium. Mean times to detection of Mycobacterium tuberculosis (n = 96) were significantly shorter with MGIT 960 (12.6 days, P = 0.003) and BACTEC 460 (11.8 days, P < 0.001) than with MB/BacT (15.9 days). Although, MGIT 960 showed the lowest rate of recovery of M. kansasii genotype I (64.3%), the earliest growth was detected with this system (8.9 days). Low and similar rates of contamination were obtained with MGIT 960 (3.3%) and MB/BacT (3%). The AccuProbe test for identification showed excellent sensitivities with MGIT 960 (96.8%) and MB/BacT (100%) cultures. In addition to being nonradiometric, both MGIT 960 and MB/BacT are accurate, rapid, and labor-saving detection systems which could replace the radiometric method.

* Corresponding author. Mailing address: Servei de Microbiologia, Hospital "Princeps d'Espanya," Ciutat Sanitària i Universitària de Bellvitge, C/Feixa Llarga s/n, 08907 L'Hospitalet de Llobregat, Barcelona, Spain. Phone: 34-93-2607930. Fax: 34-93-2607547. E-mail: falcaide{at}csub.scs.es.

Journal of Clinical Microbiology, January 2000, p. 398-401, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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