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Journal of Clinical Microbiology, January 2000, p. 424-426, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Pulsed-Field Gel Electrophoresis-Based Molecular Comparison of Vibrio cholerae O1 Isolates from Domestic and Imported Cases of Cholera in Japan

Eiji Arakawa,1 Toshiyuki Murase,2 Shigeru Matsushita,3 Toshio Shimada,1 Shiro Yamai,2 Takeshi Ito,3 and Haruo Watanabe1,*

Department of Bacteriology, National Institute of Infectious Diseases,1 and Department of Bacteriology, Tokyo Metropolitan Research Laboratory of Public Health,3 Tokyo, and Department of Bacteriology and Pathology, Kanagawa Prefectural Public Health Laboratory, Kanagawa,2 Japan

Received 7 June 1999/Returned for modification 17 August 1999/Accepted 20 September 1999

Sixty-seven Vibrio cholerae O1 El Tor isolates (36 domestic and 31 imported) were classified into 19 subtypes by NotI- and SfiI-digested pulsed-field gel electrophoresis. Twenty-five of 36 domestic and 4 imported isolates were assigned to a NotI-A1-SfiI-A1 subtype, suggesting that this pulse type is widely distributed in Asia and Japan.

* Corresponding author. Mailing address: Department of Bacteriology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. Phone and Fax: 81-3-5285-1171. E-mail: haruwata{at}nih.go.jp.

Journal of Clinical Microbiology, January 2000, p. 424-426, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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