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Journal of Clinical Microbiology, January 2000, p. 44-49, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development and Evaluation of a Phage Typing Scheme for Vibrio cholerae O139

A. K. Chakrabarti, A. N. Ghosh, G. Balakrish Nair, S. K. Niyogi, S. K. Bhattacharya, and B. L. Sarkar*

National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta-700 010, India

Received 7 May 1999/Returned for modification 22 July 1999/Accepted 27 September 1999

The scenario of cholera that existed previously changed in 1992 and 1993 with the emergence of toxigenic Vibrio cholerae O139 in India. The genesis of the new serogroup formed the impetus to search for O139 phages in and around the country. A total of five newly isolated phages lytic to V. cholerae O139 strains were used for the development of this phage typing scheme. These phages differed from each other and also differed from the existing O1 phages in their lytic patterns, morphologies, restriction endonuclease digestion profiles, and immunological criteria. With this scheme, 500 V. cholerae O139 strains were evaluated for their phage types, and almost all strains were found to be typeable. The strains clustered into 10 different phage types, of which type 1 (38.2%) was the dominant type, followed by type 2 (22.4%) and type 3 (18%). Additionally, a comparative study of phage types in 1993 and 1994 versus those from 1996 to 1998 for O139 strains showed a higher percentage of phage type 1 (40.5%), followed by type 3 (18.8%) during the period between 1993 and 1994, whereas phage type 2 (32.1%) was the next major type during the period from 1996 to 1998. This scheme comprising five newly isolated phages would be another useful tool in the study of the epidemiology of cholera caused by V. cholerae O139.

* Corresponding author. Mailing address: National Institute of Cholera and Enteric Diseases, P-33, CIT Rd., Scheme XM, Beliaghata, Calcutta-700 010, India. Phone: 91-33-350-1176. Fax: 91-33-350-5066. E-mail: icmrnicd{at}ren.nic.in.

Journal of Clinical Microbiology, January 2000, p. 44-49, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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