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Journal of Clinical Microbiology, January 2000, p. 444-447, Vol. 38, No. 1
PHLS Mycobacterium Reference Unit, Department
of Microbiology, Dulwich Public Health Laboratory, Guy's King's and
St Thomas' Medical School, Dulwich Hospital, London SE22 8QF, United
Kingdom
Received 3 August 1999/Returned for modification 10 September
1999/Accepted 28 October 1999
A blinded comparison of peptide nucleic acid-fluorescence in situ
hybridization (PNA-FISH) with routine identification methods was
performed on 74 consecutively positive mycobacterial liquid cultures.
All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81.5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with
either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium
flavescens.
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Differentiation of Mycobacterium
tuberculosis Complex and Nontuberculous Mycobacterial Liquid
Cultures by Using Peptide Nucleic Acid-Fluorescence In Situ
Hybridization Probes
*
Corresponding author. Mailing address: PHLS
Mycobacterium Reference Unit, Department of Microbiology, Dulwich
Public Health Laboratory, Guy's King's and St. Thomas' Medical
School, Dulwich Hospital, East Dulwich Grove, London SE22 8QF, United
Kingdom. Phone: 181 693 1312. Fax: 171 346 6477. E-mail:
francis.drobniewski{at}kcl.ac.uk.
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