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Journal of Clinical Microbiology, January 2000, p. 444-447, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differentiation of Mycobacterium tuberculosis Complex and Nontuberculous Mycobacterial Liquid Cultures by Using Peptide Nucleic Acid-Fluorescence In Situ Hybridization Probes

F. A. Drobniewski,* P. G. More, and G. S. Harris

PHLS Mycobacterium Reference Unit, Department of Microbiology, Dulwich Public Health Laboratory, Guy's King's and St Thomas' Medical School, Dulwich Hospital, London SE22 8QF, United Kingdom

Received 3 August 1999/Returned for modification 10 September 1999/Accepted 28 October 1999

A blinded comparison of peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) with routine identification methods was performed on 74 consecutively positive mycobacterial liquid cultures. All Mycobacterium tuberculosis cultures (48 of 48) and 22 of 27 (81.5%) nontuberculous cultures were correctly identified (including one mixed culture). Five isolates yielded no reaction with either probe and were identified as Mycobacterium xenopi, Mycobacterium fortuitum, or Mycobacterium flavescens.

* Corresponding author. Mailing address: PHLS Mycobacterium Reference Unit, Department of Microbiology, Dulwich Public Health Laboratory, Guy's King's and St. Thomas' Medical School, Dulwich Hospital, East Dulwich Grove, London SE22 8QF, United Kingdom. Phone: 181 693 1312. Fax: 171 346 6477. E-mail: francis.drobniewski{at}kcl.ac.uk.

Journal of Clinical Microbiology, January 2000, p. 444-447, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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