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Journal of Clinical Microbiology, January 2000, p. 453-455, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quality Control Guidelines for Disk Diffusion and Broth Microdilution Antimicrobial Susceptibility Tests with Seven Drugs for Veterinary Applications

Brant A. Odland,* Meredith E. Erwin, and Ronald N. Jones

Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa

Received 26 May 1999/Returned for modification 6 October 1999/Accepted 28 October 1999

This multicenter study proposes antimicrobial susceptibility (MIC and disk diffusion methods) quality control (QC) parameters for seven compounds utilized in veterinary health. Alexomycin, apramycin, tiamulin, tilmicosin, and tylosin were tested by broth microdilution against various National Committee for Clinical Laboratory Standards (NCCLS)-recommended QC organisms (Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Streptococcus pneumoniae ATCC 49619, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853). In addition, disk diffusion zone diameter QC limits were determined for apramycin, enrofloxacin, and premafloxacin by using E. coli ATCC 25922, P. aeruginosa ATCC 27853, and S. aureus ATCC 25923. The results from five or six participating laboratories produced >= 99.0% of MICs and >= 95.0% of the zone diameters within suggested guidelines. The NCCLS Subcommittee for Veterinary Antimicrobial Susceptibility Testing has recently approved these ranges for publication in the next M31 document.

* Corresponding author. Mailing address: Medical Microbiology Division, 251 MRC, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 335-8168. Fax: (319) 335-8141. E-mail: ronald-jones{at}uiowa.edu.

Journal of Clinical Microbiology, January 2000, p. 453-455, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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