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Journal of Clinical Microbiology, January 2000, p. 458-459, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Bacteremic Pneumonia Caused by a Single Clone of Streptococcus pneumoniae with Different Optochin Susceptibilities

Hsiu-Yuan Tsai,1 Po-Ren Hsueh,2,3 Lee-Jene Teng,4 Ping-Ing Lee,5,* Li-Min Huang,5 Chin-Yun Lee,5 and Kwen-Tay Luh2,3

Department of Pediatrics, Taipei Municipal Ho-Ping Hospital,1 Departments of Laboratory Medicine,2 Internal Medicine,3 and Pediatrics,5 National Taiwan University Hospital, and School of Medical Technology, National Taiwan University Medical College,4 Taipei, Taiwan

Received 6 July 1999/Returned for modification 24 August 1999/Accepted 22 September 1999

Two isolates of Streptococcus pneumoniae having different optochin susceptibilities were recovered from a blood sample of a 2-year-old boy with community-acquired pneumonia. The two isolates were documented to belong to a single clone on the basis of the isolates' identical serotype (23F), antibiograms by the E-test, random amplified polymorphic DNA patterns generated by arbitrarily primed PCR, pulsed-field gel electrophoresis, and restriction fragment length polymorphism of the penicillin-binding protein genes pbp2b and pbp2x.

* Corresponding author. Mailing address: Department of Pediatrics, National Taiwan University Hospital, No. 7, Chung-Shan South Rd., 10016 Taipei, Taiwan. Phone: 886-2-23970800-5138. Fax: 886-2-23934749. E-mail: pinging{at}ha.mc.ntu.edu.tw.

Journal of Clinical Microbiology, January 2000, p. 458-459, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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