This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nygren, M.
Right arrow Articles by Lundeberg, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nygren, M.
Right arrow Articles by Lundeberg, J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2000, p. 55-60, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Polymorphism in the Pertussis Toxin Promoter Region Affecting the DNA-Based Diagnosis of Bordetella Infection

Malin Nygren,1 Elisabet Reizenstein,2 Mostafa Ronaghi,1 and Joakim Lundeberg1,*

Department of Biotechnology, KTH, Royal Institute of Technology,1 and Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden

Received 13 May 1999/Returned for modification 21 July 1999/Accepted 27 September 1999

The pertussis toxin (PT) promoter region is a frequently used target for DNA-based diagnosis of pertussis and parapertussis infections. The reported polymorphism in this region has also allowed discrimination of species in mixtures with several Bordetella species by their specific PCR amplicon restriction patterns. In the present study, we investigated the degree of polymorphism in order to confirm the reliability of the assay. Five different sequence types of the amplified 239- or 249-bp region were found among the 33 Bordetella pertussis, B. parapertussis, and B. bronchiseptica American Type Culture Collection reference strains and patient isolates analyzed. According to the sequences that were obtained and according to the PT promoter sequences already available in the databases, restriction enzyme analysis with TaqI, BglI, and HaeII, which gave four different patterns, can be performed to reliably identify B. pertussis, B. parapertussis, and B. bronchiseptica.

* Corresponding author. Mailing address: Department of Biotechnology, KTH, Royal Institute of Technology, Teknikringen 30, 100 44 Stockholm, Sweden. Phone: 46-8-790 87 58. Fax: 46-8-24 54 52. E-mail: joakim.lundeberg{at}biochem.kth.se.

Journal of Clinical Microbiology, January 2000, p. 55-60, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Qin, X., Galanakis, E., Martin, E. T., Englund, J. A. (2007). Multitarget PCR for Diagnosis of Pertussis and Its Clinical Implications. J. Clin. Microbiol. 45: 506-511 [Abstract] [Full Text]  
  • Kamachi, K., Toyoizumi-Ajisaka, H., Toda, K., Soeung, S. C., Sarath, S., Nareth, Y., Horiuchi, Y., Kojima, K., Takahashi, M., Arakawa, Y. (2006). Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella pertussis Infection.. J. Clin. Microbiol. 44: 1899-1902 [Abstract] [Full Text]  
  • Templeton, K. E., Scheltinga, S. A., van der Zee, A., Diederen, B. M. W., Kruijssen, A. M., Goossens, H., Kuijper, E., Claas, E. C. J. (2003). Evaluation of Real-Time PCR for Detection of and Discrimination between Bordetella pertussis, Bordetella parapertussis, and Bordetella holmesii for Clinical Diagnosis. J. Clin. Microbiol. 41: 4121-4126 [Abstract] [Full Text]  
  • Reischl, U., Lehn, N., Sanden, G. N., Loeffelholz, M. J. (2001). Real-Time PCR Assay Targeting IS481 of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii. J. Clin. Microbiol. 39: 1963-1966 [Abstract] [Full Text]  
  • Farrell, D. J., McKeon, M., Daggard, G., Loeffelholz, M. J., Thompson, C. J., Mukkur, T. K. S. (2000). Rapid-Cycle PCR Method To Detect Bordetella pertussis That Fulfills All Consensus Recommendations for Use of PCR in Diagnosis of Pertussis. J. Clin. Microbiol. 38: 4499-4502 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»