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Journal of Clinical Microbiology, January 2000, p. 55-60, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Polymorphism in the Pertussis Toxin Promoter Region Affecting the DNA-Based Diagnosis of Bordetella Infection

Malin Nygren,1 Elisabet Reizenstein,2 Mostafa Ronaghi,1 and Joakim Lundeberg1,*

Department of Biotechnology, KTH, Royal Institute of Technology,1 and Department of Bacteriology, Swedish Institute for Infectious Disease Control,2 Stockholm, Sweden

Received 13 May 1999/Returned for modification 21 July 1999/Accepted 27 September 1999

The pertussis toxin (PT) promoter region is a frequently used target for DNA-based diagnosis of pertussis and parapertussis infections. The reported polymorphism in this region has also allowed discrimination of species in mixtures with several Bordetella species by their specific PCR amplicon restriction patterns. In the present study, we investigated the degree of polymorphism in order to confirm the reliability of the assay. Five different sequence types of the amplified 239- or 249-bp region were found among the 33 Bordetella pertussis, B. parapertussis, and B. bronchiseptica American Type Culture Collection reference strains and patient isolates analyzed. According to the sequences that were obtained and according to the PT promoter sequences already available in the databases, restriction enzyme analysis with TaqI, BglI, and HaeII, which gave four different patterns, can be performed to reliably identify B. pertussis, B. parapertussis, and B. bronchiseptica.

* Corresponding author. Mailing address: Department of Biotechnology, KTH, Royal Institute of Technology, Teknikringen 30, 100 44 Stockholm, Sweden. Phone: 46-8-790 87 58. Fax: 46-8-24 54 52. E-mail: joakim.lundeberg{at}biochem.kth.se.

Journal of Clinical Microbiology, January 2000, p. 55-60, Vol. 38, No. 1
0095-1137/0/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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